Koken Atelocollagen Honeycomb sponge

Code: KKN-CSH-10
  • $728.00
  • (Delivery from $95.00 )
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The 'Honeycomb' collagen sponge has a structure in which uniform pores (250-400 um) are arranged densely in one direction, into which cells can penetrate and proliferate. This structure facilitates the ready supply of nutrients to the cells inside the sponge, and releases metabolic wastes and biochemical products. Cells can proliferate and fill the lumen to form a uniform cell mass. The 'Honeycomb' collagen sponge is prepared from highly purified bovine dermal type I Atelocollagen and can be degraded by collagenase.

Suppression of tumorigenesis from transplanted mouse ES cells by cultivation with Atelocollagen Honeycomb Sponge

(M. Yamaki, Matsumoto Dental University, Japan)

Mouse ES cells cultured on honeycomb sponge were transplanted by subcutaneous injection into 6-week-old mice. Although all embryoid bodies without the honeycomb sponge formed tumor without exception, none of the embryoid bodies transplanted with the honeycomb sponge formed tumors (Fig.1).

Histological analysis of transplanted (mouse ES/+CSH) and normal kidney showed no differences between the two tissues. The results indicates normal spontaneous differentiation of the mouse ES cells in the presence of the honeycomb sponge when transplanted in vivo (Fig.2).

Product Name

Atelocollagen Honeycomb Sponge

Catalog Number



100 mg (bottle)

Sponge Dimensions

3 × 3 × 2 mm sponges
12-13 mg/mL density
200-400 µm pore size
approx. 2000 cm² / g surface area

Storage and Stability

Store at Room Temperature



Notice To Purchaser

REPROCELL is a licensed global distributor of KOKEN’s collagen-derived products everywhere, except for Japan.


Koken Co., Ltd. (Japan)

Specification Sheets

  1. Zaminy A, et al. Mesenchymal stem cells as an alternative for Schwann cells in rat spinal ord injury. (2013) Iran Biomed J. 17(3):113-122.
  2. Buser Z, et al. Inflammatory response of intervertebral disc cells is reduced by fibrin sealant scaffold in vitro. (2012) J Tissue Eng Regen Med. [Epub ahead of print]
  3. Guo Z, et al. Three-Dimensional Geometry of Honeycomb Collagen Promotes Higher Beating Rate of Myocardial Cells in Culture. (2012) Artif Organs. 36(9):816-819.
  4. Ishii I, et al. Correlation between antizyme 1 and differentiation of vascular smooth muscle cells cultured in honeycomb-like type-I collagen matrix. (2012) Amino Acids. 42(2-3):565-575.
  5. Suzuki T, et al. Growth inhibition and differentiation of cultured smooth muscle cells depend on cellular crossbridges across the tubular lumen of type I collagen matrix honeycombs. (2009) Microvasc Res. 77(2):143-149.
  6. (Japanese only) 八巻真理子 I型コラーゲンの人工細胞外マトリックスはマウス胚性幹細胞の腫瘍化能を抑制する。(2009) 日本再生医療学会雑誌 8(1):109-114.
  7. Fukushima K, et al. The axonal regeneration across a honeycomb collagen sponge applied to the transected spinal cord. (2008) J Med Dent Sci. 55(1):71-79.
  8. Imamura T, et al. Three-dimensional culture systems for differentiation of mice embryonic stem cells into hepatocyte-like cells. (2008) J Organ Dysfunct. 4(1):57-64.
  9. Kakudo N, et al. Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold. (2008) J Biomed Mater Res A. 84(1):191-197.
  10. Saeki K, et al. Highly efficient and feeder-free production of subculturable vascular endothelial cells from primate embryonic stem cells. (2008) J Cell Physiol. 217(1):261-280.
  11. Takeuchi R, et al. Low-intensity pulsed ultrasound activates the phosphatidylinositol 3 kinase/Akt pathway and stimulates the growth of chondrocytes in three-dimensional cultures: a basic science study. (2008) Arthritis Res Ther. 10(4):R77.
  12. Hidetsugu T, et al. Mechanism of bone induction by KUSA/A1 cells using atelocollagen honeycomb scaffold. (2007) J Biomed Sci. 14(2):255-263.
  13. George J, et al. Differentiation of mesenchymal stem cells into osteoblasts on honeycomb collagen scaffolds. (2006) Biotechnol Bioeng. 95(3):404-411.
  14. Imamura T, et al. Embryonic stem cell-derived embryoid bodies in three-dimensional culture system form hepatocyte-like cells in vitro and in vivo. (2004) Tissue Eng. 10(11-12):1716-1724.
  15. Itoh H, et al. A honeycomb collagen carrier for cell culture as a tissue engineering scaffold. (2001) Artif Organs. 25(3):213-217.
  16. Moriyama T, et al. Development of composite cultured oral mucosa utilizing collagen sponge matrix and contracted collagen gel: a preliminary study for clinical applications. (2001) Tissue Eng. 7(4):415-427.