EZSPHERE (Asahi Glass Corporation) EZSPHERE Microplate 6 Well (SP-polymer)

Spheroid forming low adherence plasticware. Cell/protein repellent SP-polymer coating. Thousands of micro-wells laser-etched into the surface which generates massive numbers of uniform size embryoid bodies.

Micro-well specifications: 400-500 µm diameter, 100-200 µm deep; 2400 micro-wells per well, 14,400 micro-wells per plate.

The EZSPHERE Microplate 6 well dish is a product manufactured by Asahi Glass Company, for culturing of mammalian cells and the preparation of 3D cell spheroids. Each plate is individually laser-etched and provided in a sterile, ready-to-use format. The plates are made of polystyrene material that is coated with a biocompatible non-adherent surface. Mammalian cells will "slide" to bottom of the wells by gravity and aggregate with neighboring cells according to their biologically programmed narrative. EZSPHERE products are available in a variety of sizes and microwell configurations to suit your particular needs. The plates are an excellent way of creating iPS cell EB seed stocks for transferral to bioreactors such as the ABLE Bioreactor Magnetic Stir System or other suspension culture systems. No other plates or multiwell plate configurations can give you the yields and cost-performance of an EZSPHERE plate from AGC.

Product Name

EZSPHERE Microplate 6 well (SP-polymer coating)

Catalog Number

AG4810-900SP

Size

5 plates

Storage and Stability

Store at Room Temperature

Sterility

Sterile

Notice To Purchaser

REPROCELL is a licensed AGC global distributor of EZSPHERE and EZ-Open Top Flask products everywhere, except for Japan.

Manufacturer

Asahi Glass Company (Japan)

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5. Hiroki Sato, Alimjan Idiris, Tatsuaki Miwa, Hiromichi Kumagai Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells Scientific Reports 6, Article number: 31063 (2016) doi:10.1038/srep31063
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7. Ayako Aihara, Natsuki Abe, Koichiro Saruhashi, Tatsuro Kanaki, Taito Nishino Novel 3-D cell culture system for in vitro evaluation of anticancer drugs under anchorage-independent conditions Cancer Science　Volume 107, Issue 12　December 2016 Pages 1858−1866 DOI: 10.1111/cas.13095
8. Waseem K. Raja, Alison E. Mungenast, Yuan-Ta Lin, Tak Ko, Fatema Abdurrob, Jinsoo Seo, Li-Huei Tsai Self-Organizing 3D Human Neural Tissue Derived from Induced Pluripotent Stem Cells Recapitulate AlzheimerÕs Disease Phenotypes PLOS ONE 11(9): e0161969. doi: 10.1371/journal.pone.0161969
9. Hiroyuki Koike, Ran-Ran Zhang, Yasuharu Ueno, Keisuke Sekine, Yun-Wen Zheng, Takanori Takebe, Hideki Taniguchi Nutritional modulation of mouse and human liver bud growth through a branched-chain amino acid metabolism Development 2017 144: 1018-1024; doi: 10.1242/dev.143032

Additional Publications

• Muttihi MS; Kim BJ; Jumar K; Park S; Choi UY; Han I; Park H; Lee S-H. Efficacy of matrilin-3-primed adipose-derived mesenchymal stem cell spheroids in a rabbit model of disc degeneration. Stem Cell REs Tharap 11:363 (2020).