FibColl®: high permeability atelocollagen inserts.
FibColl® Atelocollagen Insert 24 is a novel cell culture insert, sized to fit a standard 24-well plate, that uses a membrane entirely made from atelocollagen. Unlike other collagen-coated plastic membranes, FibColl® pores are not clogged, and the fiber structure of atelocollagen enables cell culture in an in vivo mimicking environment. The microporous structure between atelocollagen fibrils allows the permeation of molecules even over 600 kDa, making it suitable for barrier function assessment and co-culture models.
 Immunostaining (red: ZO-1); blue: nuclear |  TEER measurement |  TEER Results |
Epithelial cells form tight junctions that restrict the flow of ions and molecules between the luminal and basement membrane sides of the cell layer. Canine kidney tubular epithelial cells (MDCK cells) were cultured for 2 weeks on FibColl. During the course of the culture, tight junctions formed as shown the development of Transepithelial Electrical Resistance (TEER), which indicates that the free flow of ions across the epithelial barrier was impeded.
