Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF) and Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines and is absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, maintaining the properties of ES cells. The Nanog Antibody (Affinity Purified) was screened on human and mouse ES cells using immunocytochemistry and selected as the best Nanog antibody available for researchers needing to demonstrate pluripotency.
StemAb Nanog Antibody (Affinity Purified), Rabbit anti-Mouse/Human
Phosphate-buffered solution, pH 7.2, 1% BSA, 20% glycerol, and 0.01% thimerosal
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Tested by immunocytochemistry to ensure product quality.
For immunocytochemistry, the suggested use of this antibody is a 1:100 dilution. For an application specific protocol, please see Protocol: Immunocytochemistry.
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 109 and 300 of human Nanog
This antibody was purified by antigen affinity chromatography.