Oct4 (Octamer-4, also known as POU5F1) is a homeodomain transcription factor of the POU family which is involved in the regulation of pluripotency during normal development. The expression of Oct4 is associated with an undifferentiated phenotype in embryonic stem (ES) cells, while gene knockdown of Oct4 promotes differentiation. As such, it is frequently used as a marker for undifferentiated ES cells or induced pluripotent stem (iPS) cells. Oct4 is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. The Oct4 Antibody (Affinity Purified)was screened on human and mouse ES cells using immunocytochemistry and flow cytometry and selected as the best Oct4 antibody available for researchers needing to demonstrate pluripotency.
Immunocytochemistry analysis of Oct4 on H1 human ES cells. Cells were treated with StemAb Oct4 Antibody (Affinity Purified) using a 1:100 dilution followed by a secondary Cy3 conjugated antibody (red). DAPI staining was performed to visualize nuclei (blue).
Flow cytometry analysis of Oct4 on H1 human ES cells. Purple histogram represents Oct4 Antibody and open histogram represents isotype control at the same concentration.
StemAb Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human
Phosphate-buffered solution, pH 7.4, 1% BSA and 0.02% sodium azide
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Tested by immunocytochemistry and flow cytometry to ensure product quality.
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and a 1:5 dilution for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunoflourescence Staining of Cells for Flow Cytometry.
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of human Oct4
The antibody was purified by immunogen affinity chromatography.