Stemgent StemAb Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human

Oct4 (Octamer-4, also known as POU5F1) is a homeodomain transcription factor of the POU family which is involved in the regulation of pluripotency during normal development. The expression of Oct4 is associated with an undifferentiated phenotype in embryonic stem (ES) cells, while gene knockdown of Oct4 promotes differentiation. As such, it is frequently used as a marker for undifferentiated ES cells or induced pluripotent stem (iPS) cells. Oct4 is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. The Oct4 Antibody (Affinity Purified)was screened on human and mouse ES cells using immunocytochemistry and flow cytometry and selected as the best Oct4 antibody available for researchers needing to demonstrate pluripotency.

Product Name

StemAb Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human

Catalog Number

09-0023

Size

100 µL

Formulation

Phosphate-buffered solution, pH 7.4, 1% BSA and 0.02% sodium azide

Storage and Stability

Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.

Quality Control

Tested by immunocytochemistry and flow cytometry to ensure product quality.

Related Products

• mRNA Reprogramming Kit (00-0071)
• microRNA Booster Kit (00-0073)

Recommended Usage

The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and a 1:5 dilution for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunoflourescence Staining of Cells for Flow Cytometry.

Isotype

Rabbit IgG

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of human Oct4

Reactivity

Mouse, Human

Preparation

The antibody was purified by immunogen affinity chromatography.

Clone

Polyclonal

Specification Sheets

• 09-0023 Specifications Sheet

Safety Data Sheets

• 09-0023 Safety Data Sheet

Protocols

• Protocol: Immunoflourescence Staining of Cells for Flow Cytometry
• Protocol: Immunocytochemistry

1. Looijenga, L.H., Stoop, H., de Leeuw, H.P., de Gouveia Brazao, C.A., Gillis, A.J., van Roozendaal, K.E., van Zoelen, E.J., Weber, R.F., Wolffenbuttel, K.P., van Dekken, H., Honecker, F., Bokemeyer, C., Perlman, E.J., Schneider, D.T., Kononen, J., Sauter, G., Oosterhuis, J.W. POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors. Cancer Res 63: 2244-2250(2003).
2. Zaehres, H., Lensch, M.W., Daheron, L., Stewart, S.A., Itskovitz-Eldor, J., Daley, G.Q. High-efficiency RNA interference in human embryonic stem cells. Stem Cells 23: 299-305(2005).
3. Takahashi, K., and Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676(2006).
4. Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872(2007).
5. Park, I.H., Arora, N., Huo, H., Maherali, N., Ahfeldt, T., Shimamura, A., Lensch, M.W., Cowan, C., Hochedlinger, K., and Daley, G.Q. Disease-specific induced pluripotent stem cells. Cell 134: 877-886(2008).

Additional Publications

• Völkner C; Liedtke M; Patters J; Lukas J; Escobar HM; Knuebel G; Bullerdiek J; Holzmann C; Hermann A; Frech MJ. Generation of an iPSC line (AKOSi004-A) from fibroblasts of a female adult NPC1 patient, carrying the compound heterozygous mutation p.Val1023Serfs*15/p.Gly992Arg and of an iPSC line (AKOSi005-A) from a female adult control individual. Stem Cell Res 50:102127 (2021).
• Völkner C; Liedtke M; Petters J; Huth K; Knuebel G; Escobar HM; Bullerdiek J; Lukas J; Hermann A; Frech MH. Generation of an iPSC line (AKOSi006-A) from fibroblasts of a NPC1 patient, carrying the homozygous mutation p.I1061T (c.3182 T > C) and a control iPSC line (AKOSi007-A) using a non-integrating Sendai virus system. Stem Cell Res 49:102056 (2020).
• Fukunaga I; Shirai K; Oe Y; Danzaki K; Ohta SZ; Shiga T; Chen C; Ikeda K; Akamatsu W; Kawano A; Kamiya K. Generation of two induced pluripotent stem cell lines from PBMCs of siblings carrying c.235delC mutation in the GJB2 gene associated with sensorineural hearing loss. Stem Cell Res 47:101910 (2020).
• Wang K; Lin R-Z; Hong X; Ng AH; Lee CN; Neumeyer J; Wang G; Wang X; Ma M; Pu WT; Church GM; Melero-Martin JM. Robust differentiation of human pluripotent stem cells into endothelial cells via temporal modulation of ETV2 with modified mRNA. bioRxiv :https://doi.org/10.1101/2020.03.02.973289 (2020).
• Koutmani Y; Gampierakis IA; Polissidis A; Ximerakis M: Koutsoudaki PN; Polyzos A; Agrogiannis G; Karaliota S; Thomaidou D; Rubin LL; Politis PK; Karakis KP. CRH Promotes the Neurogenic Activity of Neural Stem Cells in the Adult Hippocampus. Stem Cell Rep 28:932-945.e7 (2019).
• McGrath M; Tam E; Sladkova M; AlManaie A; Zimmer M; de Peppo GM. GMP-compatible and xeno-free cultivation of mesenchymal progenitors derived from human-induced pluripotent stem cells. Stem Cell Res Therap 10:411 (2019).
• Wang L; De Solis AJ; Goffer Y; Birkenback KE; Engle SE; Tanis R; Levenson JM; Lu X; Rausch R: Purohit M; Lee J-Y; Tan J; De Rosa MC; DOege CA; Aaron HL; Martins G; Brüning JC; Egli D; Costa R; Berbari N; Leibel RL; Stratigopoulos G. Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development. JCI Insight 4:e123337 (2019).
• Baumann H; Jahn M; Muenchau A; Trilck-WInkler M; Lohmann K; Seibler P. Generation and characterization of eight human-derived iPSC lines from affected and unaffected THAP1 mutation carriers. Stem Cell Res 33:60-64 (2018).
• Sevilla A; Forero E; Zimmer M; Martinez H; Reggio K; Paull D; Egli D; Noggle S. Derivation and characterization of the NIH registry human stem cell line NYSF100 line under definde feeder-free conditions. Stem Cell Research 29:99 (2018).
• Yucer N; Holzapfel M; Jenkins Vogel T; Lenaeus L; Ornelas L; Laury A; Sareen D; Barrett R; Karlan BY; Svendsen CN. Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium. Sci Rep 7:10741 (2017).
• Um S; Lee H; Zhang Q; Kim HY; Lee J-H; Seo BM. Valproic acid modulates the mutlipotency in peridontal ligament stem cells via p53-mediated cell cycle. Tissue Engineering Regenerative Medicine 2:153 (2017.)
• Sevilla A; Forero E; Zimmer M; Martinez H; Reggio K; Paull D; Egli D; Noggle S. Derivation and characterization of the NYSCFe003-A human embryonic stem cell line. Stem Cell Res 25:217-220 (2017).
• Burnett LC; LeDuc CA; Sulsona CR; Paull D; Eddira S; Levy B; Salles JP; Tauber M; Driscoll DJ; Egli D; Leibel RL. Induced pluripotent cells (iPSC) created from skin fibroblasta of patients with Prader-Willi syndrome (PWS) retain the molecular signature of PWS. Stem Cell Res 17:526-530 (2016.)
• Sridhar A; Ohlemacher SK; Langer KB; Meyer JS. Robust differentiation of mRNA-reprogrammed human induced pluripotent stem cells toard a retinal lineage. Stem Cell Translational Medicine 5:10 (2016).
• Ohlemacher SK; Sridhar A; Xiao Y; Hochstetler AE; Sarfarazi M; Cummins TR; Meyer JS. Stepwise differentiation of retinal ganglion cells from human pluripotent stem cells enables analysis of glaucomatous neurodegeneration. _Stem Cells_34:1553 (2016).
• Cieslar-Pobuda A; Rafat M; Knoflach V; Skonieczna M; Hudecki A; Malechi A; Urasinska E; Ghavami S; Los MJ. Human induced pluripotent stem cell differentiation and direct transdifferentiatoin into corneal epithelial-like cells. Oncotarget 7:42314 (2016).
• Poleganov MA; Eminli S; Beissert T; Herz S; Moon J-I; Goldmann J; Beyer A; Heck R; Burkhart I; Roldan DB; Tureci O; Yi K; Hamilton B; Sahin U. Efficient reprogramming of human fibroblasts and blood-derived endothelial progenitor cells using nonmodified RNA for reprogramming and immune evasion. Human Gene Therapy 26:751 (2015).
• Han F; Wang W; CHen B; CHen C; LI Sen; Lu X; Duan J; Zhang Y; Zhang YA; Guo W; Li G. Human induced pluripotent stem cell-derived neurons improve motor asymmetry in a 6-hydroxydopamine-induced rat model of Parkinson's disease. Cytotherapy 17:665 (2015).
• Paull D; Sevilla A; Zhou H; Hahn AK; Kim H; Napolitano C; Tsankov A; Shang L; Krumholz K; Jagaseesan P; Woodard CM; Sun B; Vilboux T; Zimmer M; Forero E; Moroziewicz DN; Martinez H; Malicdan MCV; Weiss KA; Vensand LB; Dusenberry CR; Polus H; Sy KTL; Kahler DJ; Gahl WA; Solomon SL; Chang S; Meissner A; Eggan K; Noggle SA. Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells. Nature Methods 12:885 (2015).
• Ren J; Briones V; Barbour S; Yu W; Han Y; Tarashima M; Muegge K. The ATP binding site of the chromatin remodeling homolog LSH is required for nucleosome density and de novo DNA methylation at repeat sequences. Nucl Acids Res 43:1444 (2015).