Stemgent StemAb Sox2 Antibody (Affinity Purified), Rabbit anti-Mouse/Human

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated embryonic stem (ES) cells. Chromatin immunoprecipitation experiments have shown that Sox2 and Oct4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development. The knockdown of Sox2gene expression by siRNA in human ES cells results in loss of the undifferentiated stem cell state. Sox2 is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. The Sox2 Antibody (Affinity Purified) was screened on human and mouse ES cells using immunocytochemistry and flow cytometry and selected as the best antibody available for researchers needing to demonstrate pluripotency.

Immunocytochemistry analysis of Sox2 on R1 mouse ES cells. Cells were stained with Sox2 Antibody (Affinity Purified) using a 1:100 dilution followed by a secondary Cy3 conjugated antibody (red). DAPI staining was performed to visualize nuclei (blue).

Product Name

StemAb Sox2 Antibody (Affinity Purified), Rabbit anti-Mouse/Human

Catalog Number

09-0024

Size

100 µL

Formulation

Phosphate-buffered solution, pH 7.2, 1% BSA, and 0.1% sodium azide

Storage and Stability

Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.

Quality Control

Tested by immunocytochemistry staining to ensure product quality.

Recommended Usage

The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and a 1:5 dilution for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunoflourescence Staining of Cells for Flow Cytometry.

Concentration

0.5 mg/mL

Isotype

Rabbit IgG

Immunogen

Recombinant protein fragment containing a sequence corresponding to a region within amino acid 45 and 261 of human Sox2

Reactivity

Mouse, Human

Preparation

This antibody was purified by antigen affinity chromatography.

Clone

Polyclonal

Specification Sheets

• 09-0024 Specifications Sheet

Safety Data Sheets

• 09-0024 Safety Data Sheet

Protocols

• Protocol: Immunoflourescence Staining of Cells for Flow Cytometry
• Protocol: Immunocytochemistry

1. Rodda DJ; CHew J-L; Lim L-H; Loh Y-H; Wang B; Ng H-H; Robson P. Transcriptional Regulation of Nanog by OCT4 and SOX2. J Biol Chem 280:24731-7 (2005).
2. Boyer, L.A., Lee, T.I., Cole, M.F., Johnstone, S.E., Levine, S.S., Zucker, J.P., Guenther, M.G., Kumar, R.M., Murray, H.L., Jenner, R.G., Gifford, D.K., Melton, D.A., Jaenisch, R., and Young, R.A. (2005) Core transcriptional regulatory circuitry in human embryonic stem cells. Cell 122: 947-956.
3. Fong, H., Hohenstein, K.A., and Donovan, P.J. (2008) Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells. Stem Cell 26: 1931-1938.
4. Takahashi, K., and Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676.
5. Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.
6. Park, I.H., Arora, N., Huo, H., Maherali, N., Ahfeldt, T., Shimamura, A., Lensch, M.W., Cowan, C., Hochedlinger, K., and Daley, G.Q. (2008) Disease-specific induced pluripotent stem cells. Cell 134: 877-886.

Additional Publications

• Wang K; Lin R-Z; Hong X; Ng AH; Lee CN; Neumeyer J; Wang G; Wang X; Ma M; Pu WT; Church GM; Melero-Martin JM. Robust differentiation of human pluripotent stem cells into endothelial cells via temporal modulation of ETV2 with modified mRNA. bioRxiv :https://doi.org/10.1101/2020.03.02.973289 (2020).
• Nicaise AM; Wagstaff LJ; Willis CM; Paisie C; Chandok H; Robson P; Fossati V; Williams A; Crocker SJ. Cellular senescence in progenitor cells contributes to diminished remyelination potential in progressive multiple sclerosis. Proc Natl Acad Sci USA In press:DOI: 10.1073/pnas.1818348116 (2019).
• Wang L; De Solis AJ; Goffer Y; Birkenback KE; Engle SE; Tanis R; Levenson JM; Lu X; Rausch R: Purohit M; Lee J-Y; Tan J; De Rosa MC; DOege CA; Aaron HL; Martins G; Brüning JC; Egli D; Costa R; Berbari N; Leibel RL; Stratigopoulos G. Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development. JCI Insight 4:e123337 (2019).
• Yucer N; Holzapfel M; Jenkins Vogel T; Lenaeus L; Ornelas L; Laury A; Sareen D; Barrett R; Karlan BY; Svendsen CN. Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium. Sci Rep 7:10741 (2017).
• Paull D; Sevilla A; Zhou H; Hahn AK; Kim H; Napolitano C; Tsankov A; Shang L; Krumholz K; Jagaseesan P; Woodard CM; Sun B; Vilboux T; Zimmer M; Forero E; Moroziewicz DN; Martinez H; Malicdan MCV; Weiss KA; Vensand LB; Dusenberry CR; Polus H; Sy KTL; Kahler DJ; Gahl WA; Solomon SL; Chang S; Meissner A; Eggan K; Noggle SA. Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells. Naure Methods 12:885 (2015)
• Han F; Wang W; CHen B; CHen C; LI Sen; Lu X; Duan J; Zhang Y; Zhang YA; Guo W; Li G. Human induced pluripotent stem cell-derived neurons improve motor asymmetry in a 6-hydroxydopamine-induced rat model of Parkinson's disease. Cytotherapy 17:665 (2015)