Stemgent StemAb SSEA-3 Antibody (Purified), Rat anti-Mouse/Human

The SSEA-3 monoclonal antibody reacts with stage-specific embryonic antigen-3 (SSEA-3), a carbohydrate epitope on the major ganglioside that is expressed upon the surface of human embryonal carcinoma (EC) cells, embryonic germ (EG) cells, and pluripotent stem (PS) cells. The expression of SSEA-3 on human PS cells is down-regulated upon differentiation. In mouse, SSEA-3 is expressed on unfertilized eggs and preimplantation to early cleaved embryos, and no immunoreactivity is evident in undifferentiated murine EC, ES, and EG cells. SSEA-3 antibody can be used to characterize human ES cells and monitor their differentiation.

Product Name

StemAb SSEA-3 Antibody (Purified), Rat anti-Mouse/Human

Catalog Number

09-0014

Size

100 µL

Formulation

Phosphate-buffered solution, pH 7.2, and 0.09% sodium azide

Storage and Stability

Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.

Quality Control

Tested by immunocytochemistry and flow cytometry to ensure product quality.

Notice To Purchaser

Purchaser represents and warrants that it will use StemAb SSEA-3 Antibody (Purified), Rat anti-Mouse/Human purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Recommended Usage

The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and 0.25µg per 1 × 10⁶viable cells in 100µL for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunofluorescence Staining of Cells for Flow Cytometry.

Concentration

0.5 mg/mL

Isotype

Rat IgM, κ

Immunogen

4-8 cell stage mouse embryos

Reactivity

Mouse, Human

Preparation

This antibody was purified by affinity chromatography.

Clone

MC-631

Specification Sheets

• 09-0014 Specifications Sheet

Safety Data Sheets

• 09-0014 Safety Data Sheet

Protocols

• Protocol: Immunoflourescence Staining of Cells for Flow Cytometry
• Protocol: Immunocytochemistry

1. Kannagi, R., Cochran, N.A., Ishigami, F., Hakomori, S., Andrews, P.W., Knowles, B.B., and Solter, D. Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J2: 2355-2361 (1983).
2. Henderson, J.K., Draper, J.S., Baillie, H.S., Fishel, S., Thomson, J.A., Moore, H., and Andrews, P.W. Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337(2002).
3. Brimble, S.N., Sherrer, E.S., Uhl, E.W., Wang, E., Kelly, S., Merrill, A.H. Jr., Robins, A.J., and Schulz, T.C. The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Stem Cells 25: 54-62(2007).

Additional Publications

• Rodríguez-Traver E; Díaz-Guerra E; Rodríguez C; Arenas F; Orera M; Kulisevsky J; Moratalla R; Vicario C. A collection of three integration-free iPSCs derived from old male and female healthy subjects. Stem Cell Res 42:101553 (2020).
• Wang X; Han Y; Wang M; Bo C; Zhang Z; Xu L; Liu W; Wang H. Wnt Signaling Protects against Paclitaxel-Induced Spiral Ganglion Neuron Damage in the Mouse Cochlea In Vitro. Biomed Res Intl 2019:7878906 (2019).
• Völkner C; Peter F; Liedtke M; Krohn S; Lindner I; Escobar HM; Cimmaruta C; Lukas J; Hermann A; Frech MJ. Generation of the Niemann-Pick Type C2 patient-derived iPSC line AKOSi001-A. Stem Cell Res in press:17297 (2019).
• Secreto FJ; Li X; Smith AJ; Bruinsma ES; Perales-Clemente E; Oommen S; Hawse G; Hrstka SCL; Arendt BK; Brandt EB; Wigle DA; Nelson TJ. "Quantificaton of etoposide hypersensitivity: A sensitive, functional method for assessing pluripotent stem cell quality."Stem Cells Transl Med 6:18239 (2017).
• Kuo K-K; Lee K-T; Chen K-K; Yang Y-H; Lin Y-C; Tsai M-H; Wuputra K; Lee Y-L; Ku C-C; Miyoshi H; Nakamura Y; Saito S; Wu C-C; Chai C-Y; Eckenr R; Lin C-LSl Wang SS-W; Wu D-C; Lin C-S; Yokoyama KK. Positive feedback loop of OCT4 and c-JUN expedites cancer stemness in liver cancer. Stem Cells 34:2613-2624 (2016).