The TRA-1-60 monoclonal antibody reacts with a pluripotent-stem-cell-specific antigen expressed on undifferentiated human pluripotent stem (PS) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-60 on human PS cells is down-regulated upon differentiation.The TRA-1-60 antibody recognizes a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein, which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.
Immunocytochemistry analysis of TRA-1-60 on H1 human ES cells. Cells were stained with TRA-1-60 Antibody (Purified) at a 1:100 dilution followed by a secondary Cy 3 antibody (red). DAPI staining was performed to visualize nuclei (blue).
Flow cytometry analysis of TRA-1-60 on H1 human ES cells. Purple histogram represents TRA-1-60 Antibody (Purified) and open histogram represents isotype control at the same concentration.
StemAb TRA-1-60 Antibody (Purified), Mouse anti-Human
Phosphate-buffered solution, pH 7.2, and 0.09% sodium azide
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Tested by immunocytochemistry and flow cytometry to ensure product quality.
Notice To Purchaser
Purchaser represents and warrants that it will use StemAb TRA-1-60 Antibody (Purified), Mouse anti-Human purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and 0.25 µg per 1 × 106 viable cells in 100 µL for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunofluorescence Staining of Cells for Flow Cytometry.
Human embryonal carcinoma cell line 2102Ep
This antibody was purified by affinity chromatography.