Easy iMatrix-511 is a ready-to-use solution of iMatrix-511. Easy iMatrix-511 is useful for the culture of cells adhering to laminin-511.
iMatrix-51 is a highly purified and refined laminin-511 E8 fragments, produced in CHO-S cells. iMatrix-511 features make it an ideal matrix for pluripotent stem cell culture:
Laminin is localized to the basement membrane and plays a key role in cell adhesion and proliferation. Laminin-511 (α5, β1 laminin) binds to integrin α6β1 to promote cell signaling. Laminin-511 provides an ideal matrix for the proliferation of a wide variety of cell types including stem and iPS cells.
iMatrix-511 is functionally equivalent to Easy iMatrix-511-Silk (Cat. No. NP892-024). The only difference is the expression system used for bioproduction. The iMatrix-511 SILK product is produced in recombinant silkworm cocoon, while iMatrix-511 is produced in CHO-S cells. Both are E8 fragments that have been purified using the same processes and dissolved in PBS with HSA at a convenient working concentration.
Product Name
Easy iMatrix-511 Stem Cell Culture Substrate
Catalog Number
NP892-018
Size
Molecular Weight
150 kDa (laminin-511 E8 fragments)
Purity
>95% pure
Formulation
Recombinant iMatrix-511 E8 fragments in PBS(-) with 0.05% recombinant human serum albumin
Storage and Stability
Store at °C to 15 °C and protect from light exposure. Stable for 2 years from the manufacturing date.
Concentration
1.6 µg/mL
iMatrix Source
CHO-S cells
Quality Control
Integrin binding Kd <e; 10 nM
Sterility
Sterile
Notice To Purchaser
REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market.
Recommended Usage
Easy iMatrix-511 is suitable for use as a substrate for the culture of various cell types, including ES/iPS cells.
Manufacturer
Nippi Corporation (Japan)
