{"title":"Immortalized Cells","description":null,"products":[{"product_id":"huaec-medium-kit-for-alveolar-cells","title":"huAEC Medium Kit for Alveolar Cells","description":"\u003ch2\u003eMedium kit for immortalized human Alveolar cells.\u003c\/h2\u003e\n\u003cp\u003eThis low-serum medium is optimized for the culture of inscreenex immortalized alveolar cells. The medium kit contains 500 mL Basal Medium and 30 mL Supplement Mix, which need to be combined to form the complete medium.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFor use with Cell Lines\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCI-hAELVi Immortalized Human Alveolar Epithelial Cells (\u003ca href=\"https:\/\/www.reprocell.com\/product-catalog\/immortalized-cell-lines-and-related-reagents\/ci-haelvi-immortalized-human-alveolar-epithelial-cells\" rel=\"noopener\"\u003eINS-CI-1015\u003c\/a\u003e)\u003c\/li\u003e\n\u003cli\u003eCI-huArlo Immortalized Human Alveolar Epithelial Cells (\u003ca href=\"https:\/\/store.reprocell.com\/products\/ci-huarlo-immortalized-human-alveolar-epithelial-cells\"\u003eINS-CI-1031\u003c\/a\u003e)\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"inscreenex","offers":[{"title":"500 mL","offer_id":50648947884218,"sku":"INS-ME-1013-500ml","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/inscreenex-medium-kit-1-1024x1024.webp?v=1791202788"},{"product_id":"ci-haelvi-immortalized-human-alveolar-epithelial-cells","title":"CI-hAELVi Immortalized Human Alveolar Epithelial Cells","description":"\u003ch2\u003eImmortalized Human Alveolar Epithelial Cells\u003c\/h2\u003e\n\u003cp\u003eCI-hAELVi cells are immortalized human alveolar epithelial cells. Alveolar epithelial cells play a crucial role in the respiratory system by lining the alveoli, tiny air sacs within the lungs where gas exchange occurs. CI-hAELVis have been extensively used to model the air-blood barrier in vitro.\u003c\/p\u003e\n\u003cp\u003eWhile the cells do show an AT-1 phenotype (expression of Caveolin, no surfactant secretion) in standard culture conditions, they adopt certain AT-2 characteristics in extended ALI culture conditions, including secretion of surfactants and formation of microvilli and lamellar structures.\u003c\/p\u003e\n\u003cp\u003eThere is a newer and improved alveolar cell line available, \u003ca href=\"https:\/\/store.reprocell.com\/products\/ci-huarlo-immortalized-human-alveolar-epithelial-cells\"\u003eCI-huArlo\u003c\/a\u003e, which is a direct derivative of CI-hAELVi.\u003c\/p\u003e\n\u003cp\u003eThis CI-hAELVi cell line is the result of a collaboration between HIPS, Saarbrücken (Germany), HZI, Braunschweig (Germany), and inscreenex.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCharacteristics\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTEER of \u0026gt;1500 Ω\/sqcm in Liquid-Liquid-Interface culture\u003c\/li\u003e\n\u003cli\u003eTEER of \u0026gt;2000Ω\/sqcm in Air-Liquid-Interface culture\u003c\/li\u003e\n\u003cli\u003eTight junctions (ZO1, Occludin)\u003c\/li\u003e\n\u003cli\u003eSusceptible to SARS-CoV-2 infection\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eCulture Conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCulture Medium: huAEC Medium Kit for Alveolar Cells (Cat. No. \u003ca href=\"http:\/\/store.reprocell.com\/products\/huaec-medium-kit-for-alveolar-cells\"\u003eINS-ME-1013\u003c\/a\u003e)\u003c\/li\u003e\n\u003cli\u003eCulture Matrix: Coating Solution  (Cat. No. \u003ca href=\"https:\/\/store.reprocell.com\/products\/coating-solution\"\u003eINS-SU-1026-100ml\u003c\/a\u003e)\u003c\/li\u003e\n\u003cli\u003eCryopreservation Medium: Freezing Solution for Immortalized Cell Lines (Cat. No.\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/store.reprocell.com\/products\/freezing-medium-for-immortalized-cell-lines\"\u003eINS-SU-1027\u003c\/a\u003e\u003ca href=\"https:\/\/www.reprocell.com\/product-catalog\/immortalized-cell-lines-and-related-reagents\/freezing-medium-for-immortalized-cell-lines\" rel=\"noopener\"\u003e\u003c\/a\u003e)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eBuying and Ordering the Cells\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA license for Academic Researchers is also available.  Please \u003ca href=\"https:\/\/store.reprocell.com\/pages\/contact\"\u003econtact us \u003c\/a\u003efor more information\u003c\/p\u003e","brand":"inscreenex","offers":[{"title":"1 vial (500K cells)","offer_id":50648970297530,"sku":"INS-CI-1015","price":9430.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/inscreenex-cells-cryovials-2-1024x1024.webp?v=1791203416"},{"product_id":"ci-huarlo-immortalized-human-alveolar-epithelial-cells","title":"CI-huArlo Immortalized Human Alveolar Epithelial Cells","description":"\u003ch2\u003e\u003cspan\u003eImmortalized human alveolar epithelial cells.\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eCI-huArlo cells are immortalized human alveolar epithelial cells. The cell line is an improved version of\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/store.reprocell.com\/products\/ci-haelvi-immortalized-human-alveolar-epithelial-cells\"\u003eCI-hAELVi\u003c\/a\u003e\u003ca href=\"https:\/\/www.reprocell.com\/product-catalog\/immortalized-cell-lines-and-related-reagents\/ci-haelvi-immortalized-human-alveolar-epithelial-cells\" rel=\"noopener\"\u003e\u003c\/a\u003e alveolar epithelial cell line. It was derived from CI-huALEVi by single-cell printing, and it shows an overall improved alveolar phenotype, most notably a tighter barrier function. \u003c\/p\u003e\n\u003cp\u003eWhile the cells do show an AT-1 phenotype in standard culture conditions, they adopt certain AT-2 characteristics in extended ALI culture conditions, including secretion of surfactants and formation of microvilli and lamellar structures.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCharacteristics\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTEER of \u0026gt;1500 Ω\/sqcm in Liquid-Liquid-Interface culture\u003c\/li\u003e\n\u003cli\u003eTEER of \u0026gt;2000Ω\/sqcm in Air-Liquid-Interface culture\u003c\/li\u003e\n\u003cli\u003eTight junctions (ZO1, Occludin)\u003c\/li\u003e\n\u003cli\u003eMonolayer growth on semipermeable Inserts.\u003c\/li\u003e\n\u003cli\u003eSusceptible to SARS-CoV-2 infection\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eCulture Conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCulture Medium: huAEC Medium Kit for Alveolar Cells (Cat No. \u003ca href=\"http:\/\/store.reprocell.com\/products\/huaec-medium-kit-for-alveolar-cells\"\u003eINS-ME-1013\u003c\/a\u003e)\u003c\/li\u003e\n\u003cli\u003eCulture Matrix: Coating Solution (Cat. No. \u003ca href=\"https:\/\/www.reprocell.com\/product-catalog\/immortalized-cell-lines-and-related-reagents\/hufib-serum-free-medium-kit-for-ci-hufib-fibroblasts\" rel=\"noopener\"\u003eINS-SU-1026-100ml\u003c\/a\u003e)\u003c\/li\u003e\n\u003cli\u003eCryopreservation Medium: Freezing Solution for Immortalized Cell Lines (Cat. No. \u003ca href=\"https:\/\/store.reprocell.com\/products\/freezing-medium-for-immortalized-cell-lines\"\u003eINS-SU-1027\u003c\/a\u003e\u003ca href=\"https:\/\/www.reprocell.com\/product-catalog\/immortalized-cell-lines-and-related-reagents\/freezing-medium-for-immortalized-cell-lines\" rel=\"noopener\"\u003e\u003c\/a\u003e)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eBuying and Ordering the Cells\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA license for Academic Researchers is also available.  Please \u003ca href=\"https:\/\/store.reprocell.com\/pages\/contact\"\u003econtact us \u003c\/a\u003efor more information\u003c\/p\u003e","brand":"inscreenex","offers":[{"title":"1 vial (500 K cells)","offer_id":50649061916858,"sku":"INS-CI-1031","price":9430.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/inscreenex-cells-cryovials-2-1024x1024_92f72b63-68f5-4118-aa77-c3144a0fc8a3.webp?v=1791204906"},{"product_id":"freezing-medium-for-immortalized-cell-lines","title":"Freezing Medium for Immortalized Cell Lines","description":"\u003ch2\u003eCryopreservation medium for immortalized cells\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eThis product is a replacement for INS-SU-1004.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis freezing medium is optimized for the cryopreservation of Inscreenex immortalized cells. This freezing medium is supplied pre-mixed and ready-to-use. Freezing medium is optimized for high viability, and it contains DMSO and serum.\u003c\/p\u003e\n\u003ch3 id=\"cryopreservation-of-immortalized-cells\"\u003eCryopreservation of Immortalized Cells\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eAspirate medium.\u003c\/li\u003e\n\u003cli\u003eWash with PBS and aspirate PBS.\u003c\/li\u003e\n\u003cli\u003eAdd Trypsin\/EDTA (TE) solution to the cells and incubate at room temperature or 37 °C for 5-10min, or until the cells detach.\u003c\/li\u003e\n\u003cli\u003eExamine the cells under a microscope. When the cells start to detach, gently tap the side of the vessel to loosen the remaining cells.\u003c\/li\u003e\n\u003cli\u003eResuspend cells in 2% FBS in PBS and transfer to a 15 mL conical bottom tube.\u003c\/li\u003e\n\u003cli\u003eCentrifuge cells at 200 × g for 4 min.\u003c\/li\u003e\n\u003cli\u003eAspirate supernatant and gently resuspend the cell pellet in Freezing medium (approx. 1 million cells\/mL).\u003c\/li\u003e\n\u003cli\u003eTransfer cell suspension into cryovial(s) and place them into a freezing container (“Mr.Frosty” or similar).\u003c\/li\u003e\n\u003cli\u003ePlace the freezing container at −80 °C for 16-24 h.\u003c\/li\u003e\n\u003cli\u003eTransfer cryovials to liquid nitrogen vapor for long-term storage.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"inscreenex","offers":[{"title":"30 mL","offer_id":50649215467706,"sku":"INS-SU-1027","price":55.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/inscreenex-freezing-medium-2.jpg?v=1791207507"}],"url":"https:\/\/store.reprocell.com\/collections\/immortalized-cells.oembed","provider":"Reprocell","version":"1.0","type":"link"}