{"title":"Stem Cell Culture Media \u0026 Substrates","description":"","products":[{"product_id":"imatrix-511-silk-stem-cell-culture-substrate","title":"iMatrix 511-SILK Stem Cell Culture Substrate","description":"\u003cp\u003eRecombinant Laminin-511 E8-Fragments, expressed in Silkworm iMatrix-511 SILK is a highly purified and refined laminin-511 E8 fragments, produced in Silkworms. iMatrix-511 SILK features make it an ideal matrix for pluripotent stem cell culture: Promotes greater stem cell adhesion than all other matrix proteins that have been tested Easy to use (liquid format) E8 fragments retain integrin binding specificity and capacity, and display higher potency than natural Laminin-511 Equivalent performance, but lower cost than the legacy iMatrix-511 product Laminin is localized to the basement membrane and plays a key role in cell adhesion and proliferation. Laminin-511 (α5, β1 laminin) binds to integrin α6β1 to promote cell signaling. Laminin-511 provides an ideal matrix for the proliferation of a wide variety of cell types including stem and iPS cells. iMatrix-511 SILK  is functionally equivalent to iMatrix-511 ( Cat. No. NP892-011 ). The only difference is the expression system used for bioproduction. The iMatrix-511 SILK product is produced in recombinant silkworm cocoon, while iMatrix-511 is produced in CHO-S cells. Both are E8 fragments that have been purified using the same processes.\u003c\/p\u003e","brand":"Matrixome","offers":[{"title":"6 vials","offer_id":45980762046650,"sku":"NP892-021","price":487.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/imatrix-511-silk-stem-cell-culture-substrate-p471-392_medium_c3e9c76f-225b-4f2a-8d37-b9c5b40d9624.png?v=1781100631"},{"product_id":"imatrix-511-stem-cell-culture-substrate","title":"iMatrix 511 Stem Cell Culture Substrate","description":"\u003cp\u003eRecombinant Laminin-511 E8-Fragments iMatrix-511 is a highly purified and refined laminin-511 E8 fragments, produced in CHO cells. iMatrix-511 features make it an ideal matrix for pluripotent stem cell culture: Promotes greater stem cell adhesion than all other matrix proteins that have been tested Easy to use (liquid format) E8 fragments retain integrin binding specificity and capacity, and display higher potency than natural Laminin-511 Equivalent performance, but lower cost than the legacy iMatrix-511 product Laminin is localized to the basement membrane, and plays a key role in cell adhesion and proliferation. Laminin-511 (α5, β1 laminin) binds to integrin α6β1 to promote cell signaling. Laminin-511 provides an ideal matrix for the proliferation of a wide variety of cell types including stem and iPS cells. iMatrix-511 is functionally equivalent to iMatrix-511 SILK ( Cat. No. NP892-021 ). The only difference is the expression system used for bioproduction. The iMatrix-511 SILK product is produced in recombinant silkworm cocoon, while iMatrix-511  is produced in CHO-S cells. Both are E8 fragments that have been purified using the same processes.\u003c\/p\u003e","brand":"Matrixome","offers":[{"title":"6 vials","offer_id":45980762079418,"sku":"NP892-012","price":1006.0,"currency_code":"USD","in_stock":true},{"title":"2 vials","offer_id":46329860817082,"sku":"NP892-011","price":426.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/imatrix-511-stem-cell-culture-substrate-p455-292_medium_55149325-9c6c-4d5f-9c03-fef3a9a910c6.png?v=1781100632"},{"product_id":"nutricoat-attachment-solution","title":"NutriCoat Attachment Solution","description":"\u003cp\u003eAttachment Solution for the Serum-Free Culture of Mesenchymal Stem Cells NutriCoat™ is a defined substrate based on clinical-grade human Fibrinogen, designed for the attachment of human mesenchymal stem cells (hMSC) in serum-free (SF) and xeno-free (XF) culture systems. NutriCoat Attachment Solution is optimal for adherence of hMSC’s from multiple sources (e.g. AT, CT, BM, DP) when cells are cultured with MSC NutriStem XF Medium ( 01-0006 ) or MSC NutriStem XF Medium, Phenol Red-free ( 01-0007 ). The substrate supports the long-term culture of hMSC, as well as their enumeration using the colony-forming unit–fibroblast (CFU-F) assay under SF and XF culture conditions.  NutriCoat is a ready-to-use solution store at RT and offers a more affordable option when compared to other commonly used products.   NutriCoat™ is ideal for cell therapy research applications such as bone\/cartilage diseases, bone marrow transplants\/GVHD, cardiovascular disease,  autoimmune disease, liver disease, and cancer. Defined substrate containing clinical-grade human Fibrinogen (xeno-free) Easy-to-use stock solution for easy handling Manufactured under cGMP Suitable for both hMSC expansion and differentiation Supports long-term multi-potency of hMSC Validated for multiple sources of human MSC culture Facilitates a quick transition from research to clinical applications Note : This product is not suitable for direct administration to humans.  It is designed for use as a ancillary material to support clinical manufacturing and research.\u003c\/p\u003e","brand":"Sartorius","offers":[{"title":"1.5 mL","offer_id":45980762112186,"sku":"05-0063","price":152.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/nutricoat-attachment-solution-p520-474_medium_73d2437b-8129-4fb9-8631-286764f69ff3.png?v=1781100635"},{"product_id":"nutrifreez-d10-cryopreservation-medium","title":"NutriFreez D10 Cryopreservation Medium","description":"\u003cp\u003eNutriFreez TM D10 Cryopreservation Medium is the new name for CryoStem TM Freezing Medium NutriFreez D10 Cryopreservation Medium, formerly called CryoStem Freezing Medium (Cat. No. 01-0013-50), is a ready-to-use solution for the animal component-free, xeno-free, serum-free cryopreservation of human embryonic stem (ES), induced pluripotent stem (iPS) and mesenchymal stem cells. Pluripotent stem cells preserved with NutriFreez D10 Cryopreservation Medium result in high cell viability and recovery and express typical pluripotent markers after thawing. CryoStem Freezing Medium was developed to maintain xeno-free conditions during cryopreservation when culturing cells in a xeno-free environment. NutriFreez D10 Cryopreservation Medium has been tested on human ES cells (H1, H9 and HuES9) and mesenchymal stem cells. Superior results were obtained in comparison with both serum-containing freezing media as well as competing serum-free products, making this an ideal product for cryopreservation of valuable human stem cell cultures. Results of a cryopreservation comparison performed at the Research and Development Center for Cell Therapy (Japan) on human pluripotent stem cells (hESC and hiPSC) show that NutriFreez D10 Cryopreservation Medium presents best recovery rate for hPSCs after thawing 1 . NutriFreez D10 Cryopreservation Medium is manufactured under cGMP.\u003c\/p\u003e","brand":"Sartorius","offers":[{"title":"50 ml","offer_id":45980762177722,"sku":"01-0020-50","price":165.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/nutrifreez-d10-cryopreservation-medium-p461-368_medium_2c1a64d3-58b3-444c-abaa-2181e3618fc9.jpg?v=1781100637"},{"product_id":"nutrifreez-d10-cryopreservation-medium-phenol-red-free","title":"NutriFreez D10 Cryopreservation Medium, Phenol-Red free","description":"\u003cp\u003eA chemically defined, animal component-free, protein-free, phenol red-free cryopreservation solution NutriFreez® D10 Cryopreservation Medium is a ready-to-use, animal component-free, serum-free, and protein-free cell freezing solution optimized for multiple cell lines. When culturing cells in a serum-free environment, it is essential to also maintain serum-free conditions during cryopreservation. NutriFreez® D10 Cryopreservation Medium has been demonstrated to result in high rates of cell viability, proliferation, adherence (in relevant lines), and bioactivity\/ expression following freezing and thawing. Superior results were obtained both in comparison with serum-containing freezing media as well as competing serum-free products, making this an ideal product for both serum-containing and serum-free applications. NutriFreez® D10 Cryopreservation Medium has been extensively tested on multiple cell types, including: Human Mesenchymal Stem Cells (hMSC), from various sources (e.g. bone marrow (BM-MSC), adipose tissue (AT-MSC), umbilical cord tissue (UC-MSC) derived and dental pulp tissue (hMSC-DP) Human Embryonic Stem Cells (hESC), Induced Pluripotent Stem Cells (iPSC) Human Peripheral Blood Mononuclear Cells (PBMC's) Human Endothelial Cells (EC) T cells, including Chimeric Antigen Receptors (CART) cells and Tumor-Infiltrating Lymphocytes (TIL's) Neuron Cells Hybridomas CHO cells Vero Cells Multiple mammalian cell lines: MRC-5, HEK-293, HepG2, HeLaBSC-1, BGM3T3, MA-10BHK-21 as well as other extremely sensitive cell types NutriFreez® D10 Cryopreservation Medium Features: Cryoprotective formulation designed to minimize dehydration effects Effective maintenance of cell viability, adhesion, and bioactivity Ready-to-use solution, simple protocol Suitable for use in cell banks and biopharmaceutical applications Animal component-free, serum-free, protein-free solution cGMP Manufactured Drug Master File available\u003c\/p\u003e","brand":"Sartorius","offers":[{"title":"100 ml","offer_id":45980762210490,"sku":"01-0031-100","price":278.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/nutrifreez-d10-cryopreservation-medium-phenol-red-free-p513-459_medium_d9f740b1-af0a-45ae-8d38-80327f3141b9.jpg?v=1781100638"},{"product_id":"dissociation-solution-for-human-es-ips-cells","title":"Dissociation solution for human ES\/iPS cells","description":"\u003cp\u003eGentle detachment of the colonies without using scrapers* 10 times more viability compared to trypsin Long term culture without karyotype abnormality For both of feeder-dependent and feeder-free culture Each lot is functionally tested (Storage at −20 °C) ReproCELL's Dissociation Solution (CTK solution) has been formulated by Prof. Nakatsuji and Dr. Suemori's team at Kyoto University specifically for human ES\/iPS cells. It allows for gentle detachment of cells providing high levels of viability in subsequent passages. Dissociation Solution allows cell detachment without scraping and is ideal for use in automated protocols*. ReproCELL's Dissociation Solution has been thoroughly characterized to ensure optimal performance. High post-procedure viability of human ES\/iPS cells (10 fold higher than trypsin). [* when used in feeder-dependent culture or feeder-free culture with Laminin-5]\u003c\/p\u003e","brand":"REPROCELL","offers":[{"title":"30mL","offer_id":45980762472634,"sku":"RCHETP002","price":146.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/dissociation-solution-for-human-es-ips-cells-p363-146_medium_87830d2a-81f3-4e97-a42a-53e168c0dd5e.png?v=1781100647"},{"product_id":"msc-nutristem-xf-medium","title":"MSC NutriStem® XF Medium","description":"\u003cp\u003eFaster growth rates and stronger cell yields of hMSCs MSC NutriStem® XF Medium is a defined, serum-free, xeno-free culture medium designed for optimal growth and expansion of human mesenchymal stem\/stromal cells (hMSC) derived from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC) and umbilical cord matrix (UC-MSC). MSC NutriStem® XF Medium supports the long-term growth of hMSC while maintaining their self-renewal and multi-lineage differentiation potential. NOTE: The complete MSC culture system includes two components: this Basal Medium, and MSC NutriStem XF Supplement Mix (05-0061) . Contains Phenol Red. The basal medium is also available in a Phenol Red-free formulation (01-0007) . hMSCs cultured in MSC NutriStem XF Medium show superior proliferation and self-renewal potential in comparison to serum-containing media and other commercial serum-free media. In addition, hMSCs maintain their proper fibroblast-like cell morphology, and tri-lineage differentiation potential, and demonstrate normal hMSC marker profiles, and karyotypic stability over long-term culture. Advantages of MSC NutriStem XF Medium Serum-free, xeno-free, defined culture system Superior cell growth compared to serum-containing media Supports long-term growth and self-renewal of hMSCs from multiple sources Consistent, reliable MSC cultures Suitable for the harvest of high-quality exosomes (EVs) for future clinical applications Eliminates the need to spend time and money pre-qualifying FBS lots Manufactured under cGMP FDA Drug Master File (DMF) available Product Specifications\u003c\/p\u003e","brand":"Sartorius","offers":[{"title":"500 ml","offer_id":45980766142650,"sku":"01-0006","price":215.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/msc-nutristem-xf-medium-p514-461_medium_b736bc9c-8b67-4df6-9d86-63c2b614be40.jpg?v=1781100729"},{"product_id":"msc-nutristem-xf-medium-phenol-red-free","title":"MSC NutriStem® XF Medium, Phenol Red-free","description":"\u003cp\u003eFaster growth rates and stronger cell yields of hMSCs MSC NutriStem® XF Medium, Phenol Red-free, is a defined, serum-free, xeno-free culture medium designed for optimal growth and expansion of human mesenchymal stem\/stromal cells (hMSC) derived from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC) and umbilical cord matrix (UC-MSC). MSC NutriStem® XF Medium supports the long-term growth of hMSC while maintaining their self-renewal and multi-lineage differentiation potential. NOTEs: The complete MSC culture system includes two components: this Basal Medium, and MSC NutriStem XF Supplement Mix (05-0061) . This medium does not contain Phenol Red. The basal medium is also available in a Phenol Red-containing formulation (01-0006) . hMSCs cultured in MSC NutriStem XF Medium show superior proliferation and self-renewal potential in comparison to serum-containing media and other commercial serum-free media. In addition, hMSCs maintain their proper fibroblast-like cell morphology, and tri-lineage differentiation potential, and demonstrate normal hMSC marker profiles, and karyotypic stability over long-term culture. Advantages of MSC NutriStem XF Medium Serum-free, xeno-free, defined culture system Superior cell growth compared to serum-containing media Supports long-term growth and self-renewal of hMSCs from multiple sources Consistent, reliable MSC cultures Suitable for the harvest of high-quality exosomes (EVs) for future clinical applications Eliminates the need to spend time and money pre-qualifying FBS lots Manufactured under cGMP FDA Drug Master File (DMF) available Product Specifications\u003c\/p\u003e","brand":"Sartorius","offers":[{"title":"500 ml","offer_id":45980766175418,"sku":"01-0007","price":215.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/msc-nutristem-xf-medium-phenol-red-free-p515-463_medium_ea25bb7a-d65e-42d1-bf91-48a717ac0c36.jpg?v=1781100730"},{"product_id":"msc-nutristem-xf-supplement-mix","title":"MSC NutriStem® XF Supplement Mix","description":"\u003cp\u003eA supplement mix to be used with MSC NutriStem XF Basal Medium ( 01-0006 , 01-0007 ) Xeno-free, serum-free Designed for optimal growth and expansion of human MSCs Customizable formulation Custom scale-up services are available Scientific and regulatory support Drug Master File available\u003c\/p\u003e","brand":"Sartorius","offers":[{"title":"3 ml","offer_id":45980766208186,"sku":"05-0061","price":234.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/msc-nutristem-xf-supplement-mix-p512-457_medium_edf26f59-55b2-4621-acfa-a74c8e3304ca.jpg?v=1781100732"},{"product_id":"nutristem-hpsc-xf-medium","title":"NutriStem hPSC XF Medium","description":"\u003cp\u003eNotice to clinical users: A Drug Master File for NutriStem medium has been filed with the US FDA. Please contact us for more information. NutriStem hPSC XF Medium (formerly called NutriStem XF\/FF Culture Medium) is a fully defined xeno-free, low growth factor human embryonic stem (ES) and induced pluripotent stem (iPS) cell feeder-free culture medium that enables maintenance and expansion of pluripotent stem cells. NutriStem Medium can be used to culture pluripotent stem cells for at least 20 passages while retaining pluripotency marker expression, robust proliferation with a normal karyotype, and the ability to differentiate into cells of all three germ layers in vitro and in vivo . NutriStem Medium offers the ability to culture cells in a completely xeno-free medium without the need for high levels of basic FGF and other stimulatory growth factors and cytokines. In addition, the superior cell attachment and proliferation observed with NutriStem Medium aid high-throughput screening applications. Xeno-free, feeder-free conditions contain no animal components Easy, one-step transition from feeder-dependent culture, no adaptation required Maintains pluripotency, normal morphology, karyotype, and differentiation potential of human ES and iPS cells over long term culture Robust attachment and high cloning efficiency from single cells Morphology of Human H1 ES cells Grown in NutriStem hPSC XF Medium for 20 passages. Expression of Pluripotency Markers in hES Cells Cultured in NutriStem hPSC XF medium. Proliferation Growth Rate of Human H1 ES cells Cultured in NutriStem hPSC XF Medium versus Competitor Medium. Related Products StemRNA 3rd Gen Reprogramming Kit NutriFreez D10 Freezing Medium Product Specifications\u003c\/p\u003e","brand":"Sartorius","offers":[{"title":"500 ml","offer_id":45980766240954,"sku":"01-0005","price":362.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/nutristem-hpsc-xf-medium-p199-74_medium_b7254776-a593-4aed-b779-3f2491dca30a.jpg?v=1781100734"}],"url":"https:\/\/store.reprocell.com\/collections\/stem-cell-culture-media-substrates.oembed","provider":"Reprocell","version":"1.0","type":"link"}