Formerly called ReproNeuro.
StemRNA Neuro iPS-derived neurons are cryopreserved using REPROCELL's proprietary cryopreservation technology. Thawed and plated StemRNA Neuro cells mature during 2-3 weeks of culture on surfaces treated with Neuro Coat (RCDN201).
Note that one of the following media is required for proper maturation of the StemRNA Neuro cells:
Two different versions of StemRNA Neuro cells are currently available. Both are nearly functionally equivalent but differ in how they were derived. If you have a preference, please specify one in your order.
The dynamic modulation of Ca2+concentration in StemRNA Neuro differentiated neuronal cells can be observed with the application of a fluorescent calcium binding dye (Fluo-8). Glutamine-induced calcium flux can be observed in nearly all of the visible neurons.
Before induction
After 100 uM Glutamine induction
StemRNA Neuro cells derived from human iPS cells were first treated with high concentration Ca2+(56 uM) to polarize the cells. After polarization, increased dopamine concentration in the culture medium was detected, indicating the functional capability of dopamine release in StemRNA Neuro cells.
StemRNA Neuro cells derived from human iPS cells were treated with DMSO or DAT (Dopamine Transporter) inhibitor (GBR12909, 20 uM). Dopamine uptake was measured by Neurotransmitter transporter uptake assay kit (Molecular Devices). The fluorescence intensity of controls decreased after addition of DAT inhibitor, indicating a block of neurotransmitter transporter in ReproNeuro.
