Co-Culture kits contain GABAergic and Glutamatergic Neurons, Astroglia, and all of the media necessary to establish a co-culture model for functional neuron studies. Kits containing only GABAergic neurons, astroglia and media (Cat. No. NC1002-10), or only glutamatergic neurons, astroglia, and media (Cat. No. NC1001-10) are also available.
Co-Culture Kits are available in two configurations suitable for general culture (Cat. No. NC1010-1.5) or MEA assays (Cat. No. NC1010-7.5). Please see the specification for kit contents.
• Real Human Biology
These cells more closely resemble real human biology than alternatives such as common animal models or other iPSC-based systems. SynFire iNs provide better suitability to predict responses to test agents.
• Rapid and Homogeneous Maturation
SynFire iNs develop mature synaptic network activity within 3-4 weeks. These iNs show synchronous bursting phenotypes similar to rodent primary cultures.
• Reliable, Robust, and Ready-to-Use Cultures
This differentiation approach results in a highly defined in vitro system with lot-to-lot consistency that provides reproducible results.
• Flexible Modular System
The user can control subtype seeding densities and ratios to customize cultures to specific projects. Custom kit configurations are available.
SynFire iN (Induced Neurons) cells are suitable for a variety of biochemical assays. For example, the effect of compounds on neuronal survival, axonal outgrowth, or dendritic arborization can be measured by standard assessment of viability, or image-based analysis of labeled cells, respectively. When co-cultured with glial cells, effects on synapse formation and composition, transcriptional programs, and electrophysiology can be tested. Neuronal subtypes can be mixed in different ratios for making a defined co-culture for different experimental purposes.
Kit Contents
SynFire Co-culture Kit (Basic) (Cat. No. NC1010-1.5)
SynFire Co-culture Kit (MEA) (Cat. No. NC1010-7.5)
Storage and Stability
Store in cells in liquid nitrogen (Astroglia) or -80°C freezer (iNs) immediately upon receipt. Store other reagents at the temperature indicated on the label.
This product is stable for at least 6 months from the date of receiving when stored as directed.
QC
Functional validation of maturation and neural network formation potential of iPSC derived induced neurons has been done on MEAs. All cells have been tested for chromosomal integrity and the absence of pathogens (i.e. mycoplasma, etc). Full characterization included in the Certificate of Analysis.
Restricted Use
For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures.