SynFire (Neucyte Labs) SynFire Induced Neuron Co-Culture Kit

Human SynFire® neurons and Astroglia for co-culture studies

Co-Culture kits contain GABAergic and Glutamatergic Neurons, Astroglia, and all of the media necessary to establish a co-culture model for functional neuron studies. Kits containing only GABAergic neurons, astroglia and media (Cat. No. NC1002-10), or only glutamatergic neurons, astroglia, and media (Cat. No. NC1001-10) are also available.

Co-Culture Kits are available in two configurations suitable for general culture (Cat. No. NC1010-1.5) or MEA assays (Cat. No. NC1010-7.5). Please see the specification for kit contents.

SynFire iN (Induced Neurons) cells are suitable for a variety of biochemical assays. For example, the effect of compounds on neuronal survival, axonal outgrowth, or dendritic arborization can be measured by standard assessment of viability, or image-based analysis of labeled cells, respectively. When co-cultured with glial cells, effects on synapse formation and composition, transcriptional programs, and electrophysiology can be tested. Neuronal subtypes can be mixed in different ratios for making a defined co-culture for different experimental purposes.

 

 

 

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3-4 week old SynFire iN Co-cultures were stained with Pan-neuronal marker β3-Tubb (Tuj1) / Astroglia marker GFAP  / Nuclear staining Hoeschst, and they exhibit complex neuronal networks and morphologies and mature synaptic markers.

Patch-clamp studies show mature intrinsic and extrinsic properties in SynFire neural cultures, including action potential firing (evoked).

MEA assay of iNs co-cultured for 4 weeks using the SynFire Co-Culture Kit (MEA)(Cat. No. NC1010-7.5) shows synchrony of activity across electrodes, which is indicative of functional network formation.

Kit Contents

SynFire Co-culture Kit (Basic)    (Cat. No. NC1010-1.5)

• Part No. 1001-1.5: Glutamatergic induced neurons (>1.5x10⁶ cells/vial)
• Part No. 1002-1.5: GABAergic induced neurons (>1.5x10⁶ cells/vial)
• Part No. 1003-1.5: Astroglia (> 1.5x10⁶ cells/vial)
• Part No. 2001-10: Seeding Basal Media (Enough for 10 mL)
• Part No. 2001S-10: Seeding Supplement (Enough for 10 mL)
• Part No. 2002-20: Short-term Basal Media (Enough for 20 mL)
• Part No. 2002S-20: Short-term Supplement (Enough for 20 mL)
• Part No. 2003-60: Long-term Basal Media (Enough for 60 mL)
• Part No. 2003S-60: Long-term Supplement (Enough for 60 mL)

SynFire Co-culture Kit (MEA)    (Cat. No. NC1010-7.5)

• Part No. 1001-7.5: Glutamatergic induced neurons (>7.5x10⁶ cells/vial)
• Part No. 1002-3.5: GABAergic induced neurons (>3.5x10⁶ cells/vial)
• Part No. 1003-3.5: Astroglia (> 3.5x10⁶ cells/vial)
• Part No. 2001-20: Seeding Basal Media (Enough for 20 mL)
• Part No. 2001S-20: Seeding Supplement (Enough for 20 mL)
• Part No. 2002-40: Short-term Basal Media (Enough for 40 mL)
• Part No. 2002S-40: Short-term Supplement (Enough for 40 mL)
• Part No. 2003-120: Long-term Basal Media (Enough for 120 mL)
• Part No. 2003S-120: Long-term Supplement (Enough for 120 mL)

Storage and Stability

Store in cells in liquid nitrogen (Astroglia) or -80°C freezer (iNs) immediately upon receipt. Store other reagents at the temperature indicated on the label.

This product is stable for at least 6 months from the date of receiving when stored as directed.

QC

Functional validation of maturation and neural network formation potential of iPSC derived induced neurons has been done on MEAs. All cells have been tested for chromosomal integrity and the absence of pathogens (i.e. mycoplasma, etc). Full characterization included in the Certificate of Analysis.

Restricted Use

For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures.

Users Guides

• SynFire Users Guide
• SynFire Axion MEA Guide
• SynFire AlphaMED MED64 MEA Guide