Nippi Brightase-C

Tissue Dissociating enzyme for isolating primary cells.

Brightase-C is a recombinant Grimontia hollisae collagenase that is expressed in Brevibacillus. Unlike the commonly used C. histolyticum  collagenase, Brightase-C can cleave sequences with glutamic acid and degrade undenatured type VI collagen.  Brightase-C is highly purified to remove contaminating proteinases, yielding improved run-to-run and lot-to-lot reproducibility.

Brightase-C and Brightase-TH were used in combination to digest mouse pancreas and isolate functional beta cells.

No animal products are used in the production of Brightase-C.

Product Name

Brightase-C Recombinant Collagenase

Catalog Number

NP892-431: 40 mg

NP892-432: 2x40 mg (80 mg total)

Formulation

Lyophilized from 2 mL of a 20 mg/mL solution in 20 mM HEPES, pH 7.5, 30 mM NaCl, 2 mM CaCl₂

Storage and Stability

Store at −20 °C soon after arrival. Thaw before use.

Stock Solution

Prepare a stock solution by adding 2 mL Sterile Water per vial and agitating gently at 4 °C. Divide stock solutions into single-use aliquots and store at −20 °C. Avoid repeated freezing and thawing.

Working solutions should contain ≥1 mM CaCl₂ for optimal activity and stability.

Quality Control

• Activity: ≥6,000 U/mg
• Endotoxin: ≤10 EU/mg

Notice To Purchaser

This product is for research use only, not for therapeutic or diagnostic purposes. It is not allowed to sell this product to a third party or use it for commercial purposes without permission from REPROCELL.

Manuals

• Brightase-C datasheet

Additional Publications