Brightase-TH is a recombinant Bacillus thermoproteolyticus rokko thermolysis that is expressed in Brevibacillus. In combination with Brightase-C, Brighatse-TH provides a low endotoxin method to isolate primary cells.
No animal products are used in the production of Brightase C.
Product Name
Brightase-TH Recombinant Thermolysin
Catalog Number
NP892-441: 4.7 mg
NP892-442: 2x4.7 mg (9.4 mg total)
Formulation
Lyophilized from 5 mL of a 0.94 mg/mL solution in 50 mM HEPES, pH 7.5, 2 M NaCl, 5 mM CaCl2
Storage and Stability
Store at −20 °C soon after arrival. Thaw before use.
Stock Solution
Prepare a stock solution by adding 5 mL Sterile Water per vial and agitating gently at 4 °C. Divide stock solutions into single-use aliquots and store at −20 °C. Avoid repeated freezing and thawing.
Working solutions should contain ≥1 mM CaCl2 for optimal activity and stability.
Quality Control
Notice To Purchaser
This product is for research use only, not for therapeutic or diagnostic purposes. It is not allowed to sell this product to a third party or use it for commercial purposes without permission from REPROCELL.