Stemfactor Activin A is a crystallography-grade preparation providing the highest purity available. Activin A is bi-modal in action having been shown to maintain pluripotency of stem cells and promote differentiation1,2,3,4. Mature recombinant human Activin A is a ~25 kDa disulfide-linked homodimer of two 116 amino acid residue βA subunits.
Stemgent conveniently provides all the components comprising the 5i/L/A media supplement necessary to support naïve pluripotent stem cell applications.
L: Stemfactor LIF, Human Recombinant (03-0016), and
A: Stemfactor Activin A, Human Recombinant (03-0001)
Stemfactor Activin A, Human Recombinant
Greater than 98% by SDS-PAGE analysis
Lyophilized from 20% acetonitrile
Centrifuge briefly and reconstitute Activin A in 10 mM HCl before dilution in cell culture medium. Avoid freeze-thaw cycles as it can result in loss of activity.
Storage and Stability
Activin A is shipped at room temperature. Lyophilized Activin A is stable for up to 6 months from date of receipt when stored at −20 °C to −80 °C. Reconstituted Activin A, at concentrations greater than 0.1 mg/mL, is stable for up to 3 months when stored at −20 °C and for 1 year when stored at −80 °C.
Amino Acid Sequence
GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS
Uniprot Accession Number
P08476, residues 311-426
Less than 1.0 EU/µg of Activin A as determined by the LAL method.
The ED50 is less than 6 ng/ml as determined by its ability to inhibit the proliferation of mouse MPC-11 cells.