Stemfactor BMP-4, Human Recombinant
Stemgent

Stemfactor BMP-4, Human Recombinant

QTY Cat # Size Unit Price
03-0007 10 μg $297.00

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Overview

Bone Morphogenetic Protein 4 (BMP-4) is a polypeptide belonging to the TGFβ protein superfamily. BMP-4 is involved in bone and cartilage development; more specifically, in tooth and limb development and fracture repair1. In human embryonic development, BMP-4 is a critical signaling molecule required for the early differentiation of the embryo and establishment of a dorsal-ventral axis2,3. BMP-4 plays an important role in the differentiation of overlying ectodermal tissue. Inhibition of the BMP-4 signal causes the ectoderm to differentiate into the neural plate. In cultured stem cells, BMP-4 plays a distinct role in mouse and human embryonic stem (ES) cells. BMP-4 supports LIF as a positive factor for mouse ES cell self-renewal4. In contrast, BMP-4 induces extra-embryonic trophoblast differentiation in human pluripotent stem cells5. Stemfactor BMP-4 is a recombinant protein expressed and purified from human 293 cells as a glycosylated homodimer with a molecular mass of 34 kDa.

Specifications

Product Name: Stemfactor BMP-4, Human Recombinant

Catalog Number: 03-0007

Size: 10 µg

Purity: Greater than 95% by SDS-PAGE analysis.

Formulation: Lyophilized from sterile-filtered 1 M NaCl, 50 mM NaOAc, pH 4.5.

Reconstitution:

  • 2019 (Lot J1809-04) or earlier: Centrifuge briefly and then reconstitute BMP-4 in 4 mM HCl to yield a stock solution of no less than 0.1 mg/mL. Avoid freeze-thaw cycles as they can result in loss of activity.
  • 2020 (Lot J1912-05) or later: Centrifuge vial briefly before opening. Suspend BMP-4 in sterile water by gently pipetting water down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete suspension. For prolonged storage, dilute to a working dilution in 0.1% BSA in water, divide into single-use aliquots, and store at -80 °C. Avoid repeated freeze-thaws.

Storage and Stability: Stemfactor BMP-4 is shipped at room temperature. Lyophilized BMP-4 is stable for up to 6 months from date of receipt when stored at −20 °C to −80 °C.

  • 2019 (Lot J1809-04) or earlier: Reconstituted BMP-4, at concentrations greater than or equal to 0.1 mg/mL, is stable for up to 3 months when stored at −20 °C and up to 6 months when stored at −80 °C.
  • 2020 (Lot J1912-05) or later: Reconstituted solutions in water are stable for 1 month at 4 °C. Working dilutions in BSA solution are stable for 3 months at -20 °C to -80 °C.

Sterility: Tested to be negative for Mycoplasma sp. by PCR and microbial contamination by a sterility test.

Source: Stemfactor BMP-4 was expressed in and purified from human 293 cells.

Amino Acid Sequence: SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR

Uniprot Accession Number: P12644, residues 293-408

Endotoxin Level: Less than 1.0 EU/µg of BMP-4 as determined by the LAL method.

Biologic Activity: The ED50 is less than 30 ng/mL as determined by its ability to induce alkaline phosphatase production by mouse chondrogenic ATDC-5 cells.

Intended use: This product is intended for research use only, and is not for therapeutic or diagnostic use in humans.

Documents

1536748395sds 03 0007stemfactorbmp4humanrecombinantv1.2
References
  1. Miljkovic, N.D., Cooper, G.M., and Marra, K.G. Chondrogenesis, bone morphogenetic protein-4 and mesenchymal stem cells. Osteoarthritis Cartilage 16: 1121-1130 (2008).
  2. Chen, D., Zhao, M., and Mundy, G.R. Bone morphogenetic proteins. Growth Factors 22: 233-241 (2004).
  3. Sadlon, T.J., Lewis, I.D., and D'Andrea, R.J. BMP4: its role in development of the hematopoietic system and potential as a hematopoietic growth factor. Stem Cells 22: 457-474 (2004).
  4. Ying, Q.L., Nichols, J., Chambers, I., and Smith, A. BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3. Cell 115: 281-292 (2003).
  5. Xu, R.H., Chen, X., Li, D.S., Li, R., Addicks, G.C., Glennon, C., Zwaka, T.P., and Thompson, J.A. BMP4 initiates human embryonic stem cell differentiation to trophoblasts. Nat Biotechnol 20: 1261-1264 (2002).