Bone Morphogenetic Protein 4 (BMP-4) is a polypeptide belonging to the TGFβ protein super-family. BMP-4 is involved in bone and cartilage development; more specifically, in tooth and limb development fracture repair1. In human embryonic development, BMP-4 is a critical signaling molecule required for the early differentiation of the embryo and establishment of a dorsal-ventral axis2,3. BMP-4 plays an important role in the differentiation of overlying ectodermal tissue. Inhibition of the BMP-4 signal causes the ectoderm to differentiate into the neural plate. In cultured stem cells, BMP-4 plays a distinct role in mouse and human embryonic stem (ES) cells. BMP-4 supports LIF as a positive factor for mouse ES cell self-renewal4. In contrast, BMP-4 induces extra-embryonic trophoblast differentiation in human pluripotent stem cells5. Stemfactor BMP-4 is a recombinant protein expressed and purified from human 293 cells as a glycosylated homodimer with a molecular mass of 34 kDa.
Product Name
Stemfactor BMP-4, Human Recombinant
Catalog Number
03-0007
Size
10 µg
Purity
Greater than 95% by SDS-PAGE analysis.
Formulation
Lyophilized from sterile-filtered 1 M NaCl, 50 mM NaOAc, pH 4.5.
Reconstitution
2019 (Lot J1809-04) or earlier: Centrifuge briefly and then reconstitute BMP-4 in 4 mM HCl to yield a stock solution of no less than 0.1 mg/mL. Avoid freeze-thaw cycles as they can result in loss of activity.
2020 (Lot J1912-05) or later: Centrifuge vial briefly before opening. Suspend BMP-4 in sterile water by gently pipetting water down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete suspension. For prolonged storage, dilute to a working dilution in 0.1% BSA in water, divide into single-use aliquots, and store at -80 °C. Avoid repeated freeze-thaws.
Storage and Stability
Stemfactor BMP-4 is shipped at room temperature. Lyophilized BMP-4 is stable for up to 6 months from date of receipt when stored at −20 °C to −80 °C.
2019 (Lot J1809-04) or earlier: Reconstituted BMP-4, at concentrations greater than or equal to 0.1 mg/mL, is stable for up to 3 months when stored at −20 °C and up to 6 months when stored at −80 °C.
2020 (Lot J1912-05) or later: Reconstituted solutions in water are stable for 1 month at 4 °C. Working dilutions in BSA solution are stable for 3 months at -20 °C to -80 °C.
Sterility
Tested to be negative for Mycoplasma sp. by PCR and microbial contamination by a sterility test.
Source
Stemfactor BMP-4 was expressed in and purified from human 293 cells.
Amino Acid Sequence
SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR
Uniprot Accession Number
P12644, residues 293-408
Endotoxin Level
Less than 1.0 EU/µg of BMP-4 as determined by the LAL method.
Biologic Activity
The ED50 is less than 30 ng/mL as determined by its ability to induce alkaline phosphatase production by mouse chondrogenic ATDC-5 cells.
Intended use
This product is intended for research use only, and is not for therapeutic or diagnostic use in humans.
