Stemgent Stemfactor Hepatocyte Growth Factor, Human Recombinant

Recombinant Human Hepatocyte Growth Factor (HGF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal stem cells and targets and acts primarily upon epithelial and endothelial cells, but also acts on haemopoietic progenitor cells and T-cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration, and in wound healing.

Hepatocyte growth factor regulates cell growth, cell motility, and mophogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis and tissue regeneration.

Recombinant human HGF is an approximate 79 kDa active form of human HGF, which is a disulfide-linked heterodimer composed of α-chain (458 amino acids) and β-chain (234 amino acids) subunits. It lacks the 5 amino acid residues (F-L-P-S-S) in the 1st kringle domain; and is supplied as a glycoprotein produced by CHO cells.

Product Specifications

Product Name

Stemfactor Hepatocyte Growth Factor, Human Recombinant

Catalog Number

03-0019

Size

• 25 µg (1 µg/µL)
• 250 µg (1 µg/µL)

Purity

Greater than 99.5% by SDS-PAGE analysis.

Formulation

Dissolved in 10 mM Citrate buffer, 0.075% Tween 80, 1 M NaCl, pH 6.0. Sterilized by filtration through a 0.22 µm filter.

Storage and Stability

Store at − 70 °C.

Quality Control

Single chain form HGF <2.5% by SDS-PAGE under reducing conditions, visualized by Coomassie Brilliant Blue staining.

Sterility

Sterile filtered

Notice To Purchaser

This HGF material was produced as GMP-compatible material in a pilot scale-up run for GMP manufacturing. Custom size pharmaceutical grade GMP-compliant material is available from REPROCELL (please inquire).

Concentration

25 µL, 1 µg/µL (Cat. No. 03-0019) 250 µL, 1 µg/µL (Cat. No. 03-0019-250)

Source

Recombinant human HGF expressed in CHO cells

Endotoxin Level

Less than 0.125 EU/µg of protein as determined by the LAL method.

Biologic Activity

Biological activity was assessed based on the ability to induce proliferation of mink lung epithelial (Mv.1.Lu cells).

Manufacturer

Kringle Pharmaceutical (Japan)

• Stemfactor HGF Spec Sheet
• SDS

1. B. Rafferty, P. Maile, P. Rigsby, R.E. Gaines Das, and C.J. Robinson (2001) International standards for hepatocyte growth factor / scatter factor: initial assessment of candidate materials and their ealuation by multicentre collaborative study. Journal of Immunological Methods: 258, pp 1-11.
2. T. Seki, I. Ihara, A. Sugimura, M. Shimonishi, T. Nishizawa, O. Asam, M. Hagiya, T. Nakamura and S. Shimizu (1990) Isolation and expression of cDNA for different forms of hepatocyte growth factor from human leukocyte. Biochemical and Biophysical Research Communications, Vol. 172, No.1, pp 321-327.