Recombinant Human LIF is a lymphoid factor that promotes long-term maintenance of the pluripotency of embryonic stem cells by suppressing spontaneous differentiation1,2. Leukemia Inhibitory Factor (LIF) has additional functions such as cholinergic neuron differentiation, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo3. Human LIF is an approximate 20 kDa protein containing 202 amino acid residues and is active on both human and mouse cells.
Stemgent conveniently provides all the components comprising the 5i/L/A media supplement necessary to support naive pluripotent stem cell applications.
5i (inhibitors):
L: Stemfactor LIF, Human Recombinant (03-0016), and
A: Stemfactor Activin A, Human Recombinant (03-0001)
Product Name
Stemfactor LIF, Human Recombinant
Catalog Number
03-0016
Size
1mL, 10µg/mL 1mL, 100µg/mL
Purity
Greater than 99% by SDS-PAGE analysis.
Formulation
LIF is supplied as a 0.22µm sterile filtered liquid in PBS containing 1% w/v BSA as carrier.
Storage and Stability
Store LIF at 4°C. Do not freeze. Stable for up to 6 months from date of receipt, when stored as directed.
Concentration
1mL, 10µg/mL (Cat. No. 03-0016) 1mL, 100µg/mL (Cat. No. 03-0016-100)
Source
E. coli
Endotoxin Level
Less than 1 EU/µg of LIF as determined by the LAL method.
Biologic Activity
Human LIF activity is assessed by its ability to induce differentiation of M1 myeloid leukemia cells. The specific activity is 1 × 106units/mL (Cat. No. 03-0016) and 1 × 107units/mL (Cat. No. 03-0016-100), where 50 units is defined as the amount of human LIF required to induce differentiation in 50% of the M1 colonies in 1mL of medium.
