Stemgent Stemfactor LIF, Mouse Recombinant

Mouse LIF is a lymphoid factor that promotes long-term maintenance of the pluripotency of mouse embryonic stem cells by suppressing spontaneous differentiation^1,2. Leukemia Inhibitory Factor (LIF) has additional functions such as cholinergic neuron differentiation, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Mouse LIF is an approximate 20 kDa protein containing 202 amino acid residues.

Product Name

Stemfactor LIF, Mouse Recombinant

Catalog Number

03-0011

Size

• 1 mL, 10 µg/mL (03-0011)
• 1 mL, 100 µg/mL (03-0011-100)

Purity

Greater than 98% by SDS-PAGE analysis.

Formulation

LIF is supplied as a 0.22 µm sterile filtered liquid in PBS containing 1% w/v BSA as carrier.

Storage and Stability

LIF is shipped at room temperature. Concentrated LIF is stable for up to 6 months from date of receipt when stored at 4 °C. Prior to use dilute LIF in sterile tissue culture media, aliquot to a convenient concentration, and store at 4 °C. Avoid freeze-thaw cycles as it can result in loss of activity.

Sterility

Tested to be negative for mycoplasma by PCR and microbial contamination by a sterility test.

Recommended Usage

• 1 mL of mouse LIF with the specific activity of 1×10⁶ units/ml is sufficient to treat 1 L of embryonic stem cell culture media.
• 1 mL of mouse LIF with the specific activity of 1×10⁷ units/ml is sufficient to treat 10 L of embryonic stem cell culture media.

Concentration

• 10 µg/mL (Cat. No. 03-0011)
• 100 µg/mL (Cat. No. 03-0011-100)

Source

E. coli

Endotoxin Level

Less than 1 EU/µg of LIF as determined by the LAL method.

Biologic Activity

Mouse LIF activity is assessed by its ability to induce differentiation of M1 myeloid leukemia cells. The specific activity is 1×10⁶ units/mL (Cat. No. 03-0011) and 1×10⁷ units/mL (Cat. No. 03-0011-100), where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1 mL of medium.

Specification Sheets

• 03-0011 Specifications Sheet
• 03-0011-100 Specifications Sheet

Safety Data Sheets

• 03-0011 and 03-0011-100 Safety Data Sheet

1. Niwa, H., Ogawa, K., Shimosato, D., and Adachi, K.) A parallel circuit of LIF signalling pathways maintains pluripotency of mouse ES cells. Nature 460: 118-122 (2009).
2. Xu, J., Wang, F., Tang, Z., Zhan, Y., Zhang, J., Yan, Q., Xiong, Y., Xie, X., Wu, J., and Zhang, S. Role of leukaemia inhibitory factor in the induction of pluripotent stem cells in mice. Cell Biol Int 34: 791-797 (2010).
3. Fukunaga, N., Teramura, T., Onodera, Y., Takehara, T., Fukuda, K., and Hosoi, Y. Leukemia inhibitory factor (LIF) enhances germ cell differentiation from primate embryonic stem cells. Cell Reprogram 12: 369-376(2010).
4. Metcalf D. The unsolved enigmas of leukemia inhibitory factor. Stem Cells 21: 5-14 (2003).
5. Williams, R.L., Hilton, D.J., Pease, S., Willson, T.A., Stewart, C.L., Gearing, D.P., Wagner, E.F., Metcalf, D., Nicola, N.A., and Gough, N.M. Myeloid leukemia inhibitory factor maintains the developmental potential of embryonic stem cells. Nature 336: 684-687(1988).
6. Metcalf, D., Hilton, D.J., and Nicola, N.A. Clonal analysis of the actions of the murine leukemia inhibitory factor on leukemic and normal murine hemopoietic cells. Leukemia 2: 216-221(1988).

Additional Publications

• Huang G; Yan H; Ye S; Tong C; Ying Q-L. STAT3 Phosphorylation at Tyrosine 705 and Serine727 Deferentially Regulates Mouse ESC Fates. Stem Cells 32:1149-1160 (2014).
• Ying, Q., Tai, C. Gbx2, a LIF/Stat3 target, promotes reprogramming to and retention of the pluripotent ground state. Journal of Cell Science 126, 1093 — 1098 (2013).
• Li P; Chen L; Mak KK; Wong CK; Want CC; Yuan P. Functional Role of Mst1/Mst2 in Embryonic Stem Cell Differentiation. PLoS One 8:e79867 (2013).