Growth of iPS cells in spheroid suspension culture (3D) closely resemble embryoid body structures naturally formed by embryonic stem cells. This 3D growth format offers improved yield, viability and high efficiency for the expansion of human iPS stem cells and differentiation in a cost-effective format. The ABLE 3D Magnetic Stir and Disposable Bioreactor System enables stem cell researchers to utilize a 100 mL bioreactor vessel for cell culturing or production of up to 2 × 108 cells per vessel (or more), equivalent to about forty 10 cm plates.
The ABLE 100 mL disposable bioreactor works with the ABLE Biott ABLE Bioreactor Magnetic Stir System (30 mL) (Cat. No. ABBWSS03N0S-6) to provide affordable bioreactor capabilities to laboratory scale projects.
The 100 mL disposable bioreactor features a delta-wing-shaped impeller with a magnet on each blade located within the bioreactor, which provides low-shear agitation. This unique impeller design encourages the formation and growth of uniform spheroid cell clusters. The bioreactor allows for the flexibility of using seed stock from single-cell suspensions of iPS stem cells, passaged colony fragments, or mini-spheroids generated from low-adherence cell culture dishes or multi-well plates.
