Growth of iPS cells in spheroid suspension culture (3D) closely resembles embryoid body structures naturally formed by embryonic stem cells. This 3D growth format offers improved yield, viability, and high efficiency for the expansion of human iPS cells and differentiation in a cost-effective format. The ABLE 3D Magnetic Stir and Disposable Bioreactor System enable stem cell researchers to utilize a 30 mL bioreactor vessel for cell culturing or production of up to 5 x 107 cells per vessel, equivalent to approximately ten 10 cm plates.
The ABLE 30 mL disposable bioreactor works with the ABLE Biott ABLE Bioreactor Magnetic Stir System (30 mL) (Cat. No. ABBWSS03N0S-6) to provide affordable bioreactor capabilities to laboratory-scale projects.
ABLE Bioreactor Flask contains a delta-wing-shaped impeller with a magnet on each blade located within the bioreactor provides low-shear agitation. Its design encourages the formation and growth of uniform spheroid cell clusters. The bioreactor allows for the flexibility of using seed stock from single-cell suspensions of iPS cells, passaged colony fragments, or mini-spheroids generated from low-adherence cell culture dishes or multi-well plates.
Bioreactor system features
