Ajinomoto StemFit Basic02

StemFIt Basic 02 is a cell culture medium optimized for iPS cell expansion and maintenance. This medium supports cultivation of cells in both attached and suspension format. The medium also enables single cell passaging of iPSCs. We recommended supplementing the medium with Y27632 Rock Inhibitor; Cat. No. 04-0012. For complete medium preparation, two separate components need to be mixed, and the addition of bFGF (03-0002) is also required (not supplied with this product).

StemFit ASB01 medium is configured for sale in the USA and European market. Please contact us for a configuration sold in Japan and Asia. This product is manufactured by Ajinomoto Company (Japan).

Product Name

StemFit Basic 02 Cell Culture Medium

Catalog Number

ASB01

Size

500 mL

Storage and Stability

Store components separately as indicated (−20 °C); store reconstituted medium at 4 °C

Sterility

Sterile

Related Products

StemFit AK02N medium is a functionally equivalent formulation sold in Japan.

Recommended Usage

For cultivation of stem cells or iPSCs

System Components

The product consists of 2 components; a base medium (400 mL), and an additive (100 mL)

Manufacturer

Ajinomoto Co. (Japan)

Safety Data Sheets

• Ajinomoto StemFitBasic02 (Medium for Stem Cell)

1. Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S. A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Sci Rep 2014 Jan 8;4:3594. doi: 10.1038/srep03594 (2014).
2. Daisuke Doi, et al.Isolation of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation Stem Cell Reports Mar 11; 2(3): 337-350 (2014).
3. Takuji Kawamura, et al. Cardiomyocytes Derived from MHC-Homozygous Induced Pluripotent Stem Cells Exhibit Reduced Allogeneic Immunogenicity in MHC-Matched Non-human Primates. Stem Cell ReportsMar 8; 6(3): 312-320 (2016).
4. Akihiro Arakawa, et al. Speciation of Intracellular Zn, Fe and Cu within both iPS Cells and Differentiated Cells Using HPLC Coupled to ICP-MS. J Anal Bioanal Tech 7:295 (2016).
5. Taizo Ishikawa, et al. Genetic and pharmacological correction of aberrant dopamine synthesis using patient iPSCs with BH4 metabolism disorders. Human Molecular Genetics 25:5188 (2016).
6. Naoki Ichiyanagi, et al. Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells. Stem Cell Reports. Apr 12; 6(4): 496-510 (2016).
7. Shin-Il Kim, et al. Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods Mol Biol 1357:111-31 (2016).

Additional References

• Sugawara T; Miura T; Kawasaki T; Umezawa A; Akutsu H. The hsa-miR-302 cluster controls ectodermal differentiation of human pluripotent stem cell via repression of DAZAP2. Regen Therap 15:1-9 (2020).