c-Myc mRNA, Human

Stemgent c-Myc mRNA encodes the c-Myc protein commonly used in cellular reprogramming systems for the generation of induced pluripotent stem (iPS) cells. This_in vitro_transcribed mRNA incorporates both pseudouridine and 5-methylcytidine modified nucleotides to minimize the cellular interferon response to single stranded RNA. 1,2 Repeated transfection of c-Myc mRNA in combination with mRNAs encoding Oct4, Klf4, Sox2 and Lin-28 has been shown to reprogram human fibroblasts. 2 Figure 1 : Protein expression and localization shown by immunocytochemistry, DAPI in blue, c-Myc in red.