inscreenex
Freezing Medium for Immortalized Cell Lines
| QTY | Cat # | Size | Unit Price |
|---|---|---|---|
|
|
INS-SU-1027 | 30 mL | $55.00 |
* Prices listed are in USD. Taxes, shipping & handling not included. Contact us for bulk pricing.
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Overview
Cryopreservation medium for immortalized cells
This product is a replacement for INS-SU-1004.
This freezing medium is optimized for the cryopreservation of Inscreenex immortalized cells. This freezing medium is supplied pre-mixed and ready-to-use. Freezing medium is optimized for high viability, and it contains DMSO and serum.
Cryopreservation of Immortalized Cells
- Aspirate medium.
- Wash with PBS and aspirate PBS.
- Add Trypsin/EDTA (TE) solution to the cells and incubate at room temperature or 37 °C for 5-10min, or until the cells detach.
- Examine the cells under a microscope. When the cells start to detach, gently tap the side of the vessel to loosen the remaining cells.
- Resuspend cells in 2% FBS in PBS and transfer to a 15 mL conical bottom tube.
- Centrifuge cells at 200 × g for 4 min.
- Aspirate supernatant and gently resuspend the cell pellet in Freezing medium (approx. 1 million cells/mL).
- Transfer cell suspension into cryovial(s) and place them into a freezing container (“Mr.Frosty” or similar).
- Place the freezing container at −80 °C for 16-24 h.
- Transfer cryovials to liquid nitrogen vapor for long-term storage.