iMatrix-111 Hepatoblast-Like Epithelial Cell Culture Substrate

Highly purified and refined human recombinant laminin-111 (E8 fragment) expressed by CHO-S cells

 iMatrix-111 is a highly purified and refined product of human recombinant laminin-111 (E8 fragment) expressed by CHO-S cells. Laminin 111 supports the survival, proliferation, and differentiation of many different cell types in vitro. However, its distribution after birth is restricted to only a few tissues, such as the brain and kidney. Many of the cultivated cell types would not encounter this protein naturally in vivo, and it is advisable to match your specific cell type to the laminin naturally expressed by this cell, improving cell culture. During development, Reichert’s membrane supporting the extraembryonic trophoblasts expresses laminin 111. Reichert’s membrane is essential for epiblast differentiation, which is essential for the formation of the three embryonic germ layers by pluripotent cells in the inner cell mass.

In the adult, laminin α1 chain expression is relatively restricted and is only present in some epithelial basement membranes, and it is primarily found in tissues such as the eye, liver, and kidney, whereas it is hardly expressed at all in endothelial, adipose, nervous, or muscle tissues.