iMatrix-211 Cardiac and Myoblast Cell Culture Substrate
Matrixome

iMatrix-211 Cardiac and Myoblast Cell Culture Substrate

QTY Cat # Size Unit Price
NP892-061 175 μg × 2 tubes $509.00
NP892-062 175 μg × 6 tubes $1,418.00

* Prices listed are in USD. Taxes, shipping & handling not included. Contact us for bulk pricing.

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Overview

 Xeno-free, recombinant Laminin-221 E8 Fragment expressed in CHO-S cells

iMatrix-221 is a highly purified and refined product of human recombinant laminin-221 (E8 fragment) expressed by CHO-S cells. Laminin-221 is found predominantly in the basal lamina of striated muscle tissues. Expression deficiencies are implicated in muscular dystrophy and cardiomyopathies.1

iMatrix-221's features make it an ideal matrix for some cell culture applications:

  • Xeno-free formulation / CHO-S cell bioproduction
  • Easy to use (liquid format)
  • E8 fragments retain integrin binding specificity and capacity, and display higher potency than natural Laminin-221

Laminins of the α-2 type are also a predominant form found in the human adult heart tissue.2 Cultivation of iPSC-derived cardiomyocytes on iMatrix-221 has been demonstrated to maintain strong contractility in attached monolayer culture.3

Specifications

Product Name: iMatrix-221 Cardiac and Myoblast Cell Culture Substrate

Catalog Numbers:

  • NP892-061
  • NP892-062

Sizes:

  • NP892-061 — 2 × 175 µg
  • NP892-062 — 6 × 175 µg

Molecular Weight: 150 kDa

Purity: > 95 % pure

Formulation: Purified Laminin-221 E8 proteolytic fragment

Storage and Stability: Store at 4 °C and protect from light exposure. Stable for 2 years from manufacturing date.

Quality Control: Activity: Kd for integrin binding

Sterility: Sterile

Notice To Purchaser: REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market.

Recommended Usage: iMatrix-221 is suitable for use as a substrate for culture of various myoblast cell types, but most notably for cardiomyocytes.

Concentration: 500 µg/mL

Source: CHO-S cell expression

Manufacturer: Matrixome Corporation (Japan)

References
  1. Oliviero P. et.al., Expression of laminin α2 chain during normal and pathological growth of myocardium in rat and human. Cardiovascular Research 46, 346-355 (2000).
  2. Ja K.P. Myu Mia et.al., PSC-derived human cardiac progenitor cells improve ventricular remodeling via angiogenesis and interstitial networking of infarcted myocardium. J Cell Mol Med 20(2), 323-332 (2016).
  3. Unpublished, Osaka University (2018).