iMatrix-411 Endothelial Cell Substrate
Matrixome

iMatrix-411 Endothelial Cell Substrate

QTY Cat # Size Unit Price
NP892-041 175 μg × 2 tubes $509.00
NP892-042 175 μg × 6 tubes $1,418.00

* Prices listed are in USD. Taxes, shipping & handling not included. Contact us for bulk pricing.

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Overview

High purity, recombinant human Laminin-411 E8 Fragment expressed in CHO-S cells. 

Laminin-411 is found predominantly in the vascular endothelial basement membrane. Stem cells cultivated on iMatrix-411 are robustly induced to differentiate into endothelial progenitor cells.

iMatrix-411 features make it an ideal matrix for some cell culture applications:

  • Xeno-free formulation / CHO-S cell bioproduction
  • Easy to use (liquid format)
  • E8-fragments retain integrin binding specificity and capacity, and display higher potency than natural Laminin-411
  • Cultivation of iPSC on iMatrix-411 induces a cell fate transition to endothelial progenitor cells

iMatrix-411 is a highly purified and refined product of human recombinant laminin-411 (E8 fragment) expressed by CHO-S cells. Laminin-411 is found predominantly expressed in the vascular endothelial basement membrane1 and in Langerhans islet capillary endothelial cells of the pancreas.2

As a cell culture ECM substrate, iMatrix-411 can assist in establishing human endothelial cell primary cultures from fresh human bio-specimens.

Human iPS cells, when cultured on iMatrix-411, are robustly induced (>95%) to differentiate into endothelial progenitor cells with angiogenesis capacity.1 Other published reports have used iMatrix-411 and iMatrix-511 in a step-wise protocol to generate cholangiocytes capable of forming 3D cyst-structures from iPSC-derived hepatoblastic cells.3

Specifications

Product Name: iMatrix-411 Endothelial Cell Substrate

Catalog Numbers:

  • NP892-041
  • NP892-042

Sizes:

  • NP892-041: 2 × 175 µg
  • NP892-042: 6 × 175 µg

Molecular Weight: 150 kDa

Purity: > 95 % pure

Formulation: Purified Laminin-411 E8 proteolytic fragment

Storage and Stability: Store at 4 °C and protect from light exposure. Stable for 2 years from manufacturing date.

Quality Control: Activity: Kd for integrin binding

Sterility: Sterile

Notice To Purchaser: REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market

Recommended Usage: iMatrix-411 is suitable for use as a substrate for culture of various cell types, but most notably for vascular endothelial cells.

Concentration: 500 µg/mL

Source: CHO-S cell expression

Manufacturer: Matrixome Corporation (Japan)

References
  1. Ohta R. et al., "Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells". Scientific Reports 6:35680 / DOI: 10.1038/srep35680 (2016).
  2. Lammert E.,Cleaver O.,Melton D., Mechanisms of Development (Elsevier)120 (1) 59-64 (2003).
  3. Takayama K. et al., "Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells". Biochemical and Biophysical Research Commun. 474 (1): 91-96 (2016).