Sartorius

NutriFreez D10 Cryopreservation Medium, Phenol-Red free

QTY	Cat #	Size	Unit Price
	01-0031-100	100 mL	$278.00

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Overview

A chemically defined, animal component-free, protein-free, phenol red-free cryopreservation solution

NutriFreez® D10 Cryopreservation Medium is a ready-to-use, animal component-free, serum-free, and protein-free cell freezing solution optimized for multiple cell lines. When culturing cells in a serum-free environment, it is essential to also maintain serum-free conditions during cryopreservation.

NutriFreez® D10 Cryopreservation Medium has been demonstrated to result in high rates of cell viability, proliferation, adherence (in relevant lines), and bioactivity/ expression following freezing and thawing. Superior results were obtained both in comparison with serum-containing freezing media as well as competing serum-free products, making this an ideal product for both serum-containing and serum-free applications.

NutriFreez® D10 Cryopreservation Medium has been extensively tested on multiple cell types, including:

Human Mesenchymal Stem Cells (hMSC), from various sources (e.g. bone marrow (BM-MSC), adipose tissue (AT-MSC), umbilical cord tissue (UC-MSC) derived and dental pulp tissue (hMSC-DP)
Human Embryonic Stem Cells (hESC), Induced Pluripotent Stem Cells (iPSC)
Human Peripheral Blood Mononuclear Cells (PBMC's)
Human Endothelial Cells (EC)
T cells, including Chimeric Antigen Receptors (CART) cells and Tumor-Infiltrating Lymphocytes (TIL's)
Neurons
Hybridomas
CHO cells
Vero Cells
Multiple mammalian cell lines: MRC-5, HEK-293, HepG2, HeLaBSC-1, BGM3T3, MA-10BHK-21, as well as other extremely sensitive cell types

NutriFreez® D10 Cryopreservation Medium Features:

Cryoprotective formulation designed to minimize dehydration effects
Effective maintenance of cell viability, adhesion, and bioactivity
Ready-to-use solution, simple protocol
Suitable for use in cell banks and biopharmaceutical applications
Animal component-free, serum-free, protein-free solution
cGMP Manufactured
Drug Master File available

Specifications

Product Name

NutriFreez D10 Cryopreservation Medium, Phenol Red-free

Catalog Number

01-0031-100(REPROCELL)
05-714-1B (Sartorius)

Size

100 mL

Storage and Stability

Store NutriFreez D10 Cryopresrervation Medium, Phenol red-free at 2-8 °C. Medium is stable until expiration date listed on the bottle if stored as indicated.

Quality Control

Each lot is tested for pH, sterility, and recovery and morphology of Vero cells, and human stem cells (hPCS and hMSC). For full specifications please check the lot-specific Certificate of Analysis (CoA).

Sterility

Sterile

Quality Assurance

For in vitro diagnostic use, research use or for use as ancillary material in manufacturing Cell-Gene or Tissue-based products.
Notified under US FDA IVD part 864.9225 Cell-freezing apparatus and reagents for in vitro diagnostic use.
Listed in Europe under CE IVD class I, thus comply with European In-Vitro Diagnostic Devices Directive (98/79/EC) requirements.
Manufactured under ISO 13485 QMS and in compliance with applicable cGMP guidelines.
Manufactured under controlled environments and processes per:
- ISO 13408 – Aseptic Processing of Health Care Products;
- ISO 14644 – Airborne Particulate Cleanliness Classes in Clean Rooms and Clean Zones.

Related Products

NutriFreez D10 Cryopreservation Medium (01-0020-50)

Notice To Purchaser

REPROCELL is an authorized global distributor of select cell culture products from Sartorius.