Stemgent

StemEdit hiPSC CIITA KO RPC-LLF-34-F3

Cat # Size
RCRP051 1 Vial
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Overview

Ready-to-use Research-Grade HLA Class II knockout iPSCs made using REPROCELL’s RNA Reprogramming Technology.

These cells feature all the advantages of our RNA reprogramming technology, including no retention of reprogramming factors and easy culture in NutriStem hPSC XF Medium. They have been engineered using OpenCRISPR-1 to contain a CIITA (HLA Class II)  knockout mutation, which is carefully characterized and offers significant time savings compared to creating your own reprogrammed and edited cell lines.

The parent line, StemRNA Clinical iPSC Clone LLF-34-F3, is also available for clinical and research use.  Please contact us for more info,

 

Cat. No.: RCRP051
Parent Clone ID StemRNA Clinical iPSC Clone LLF-34-F3
Donor Race: Caucasian
Donor Sex: Female
Donor Age: 22
Reprogramming Technology: StemRNA Reprogramming Technology
Tissue Source: Skin (Fibroblasts)
Karyotype: Normal (46 XX)
Microbiology: Negative for bacteria, virus, mycoplasma

Specifications

Product Name: StemEdit hiPSC CIITA KO RPC-LLF-34-F3

Product Description: Human iPS Cells established from fibroblasts, reprogrammed with REPROCELL's StemRNA Reprogramming technology, with knockout in the CIITA (HLA Class II) gene.

Parental Clone ID: StemRNA Clinical iPSC Clone LLC-34-F3

Tissue Source: Skin (fibroblasts)

Catalog Number: RCRP051

Donor Information

  • Race: Caucasian
  • Sex: Female
  • Age: 22 years old

Precryopreservation Culture Conditions

  • Culture Medium: NutriStem hPSC XF Medium (01-0005)
  • Culture Matrix: iMatrix-511 (Cat. No. NP892-011)
  • Cryopreservation Medium: NutriFreez™ D10 Freezing Medium (Cat. No. 01-0020-50)

Size: 1 vial, 1 × 10^6 cells per vial

Storage: Store in liquid nitrogen vapor phase.

Quality Control

  • Pluripotency by ICC, flow cytometry: Pass
  • Knockout verification by NGS: Pass
  • Viability: > 70 %
  • Bacteria: Negative
  • Mycoplasma: Negative
  • Virus (HIV1&2, HBV, HCV): Negative
  • Karyotype: 46, XX

Sterility: Sterile

Notice to Purchaser: This product is intended for Research Use Only. Commercial Use may require additional agreements. Please contact info-us@reprocell.com for more information. This Product is not intended for Clinical or Diagnostic Use.