{"product_id":"stemedit-oc-1-protein","title":"StemEdit OC-1 Protein","description":"\u003ch2 id=\"opensource-genome-editing-for-research\"\u003e\u003cstrong\u003eOpen-Source Genome Editing for Research\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStemEdit OC-1 Protein is a recombinant, AI-designed genome-editing nuclease that provides a next-generation alternative to conventional Cas nucleases. StemEdit OC-1 Protein enables high editing efficiency with low off-target activity while helping researchers reduce intellectual property barriers.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp\u003eEach vial provides 130 µg of StemEdit OC-1 Protein, suitable for 2-3 assays under typical conditions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhy Choose StemEdit OC-1?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eOpen-Source Technology\u003c\/strong\u003e\u003cbr\u003e \u003cspan\u003eBased on open-source OpenCRISPR-1™ (OC-1) technology, providing an alternative to conventional Cas nucleases.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Editing Efficiency\u003cbr\u003e\u003c\/strong\u003eRobust genome editing performance across a wide range of cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLow Off-Target Activity\u003cbr\u003e\u003c\/strong\u003eDesigned for precise and reliable genome modification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAI-Designed Technology\u003cbr\u003e\u003c\/strong\u003eEngineered using advanced AI-guided protein design for next-generation genome editing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003eStemEdit OC-1 Protein was devised using large protein language models (LLMs) pre-trained on millions of naturally occurring CRISPR–Cas sequences to learn the underlying principles of functional genome editors. These models were then fine-tuned specifically on CRISPR–Cas data to guide generation toward proteins with the structural and functional characteristics of Cas9-like nucleases, creating novel gene editors far outside the range of sequences found in nature. OC-1 was identified from millions of sequences based on its strong editing activity, on-target efficiency comparable to traditional SpCas9, and reduced off-target effects in human cells, making it well-suited for genome engineering applications in stem cell and cell therapy research.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTypical experimental conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIn a typical experiment using iPSCs and a guide RNA (gRNA) specific to the desired edit, an OC-1\/gRNA ribonucleoprotein complex (RNP) was formed at a 2:1 to 4:1 mole ratio of gRNA to OC-1 protein, incubated for 10 min at room temperature, mixed with cell suspension (1 million cells in 100 uL suspension), and delivered to the cells using the Neon NxT Electroporation System (Thermo Fisher). Buffers provided in the Neon NxT kit were used to form the RNP and electroporate the cells. For iPSCs, we typically use 3-5 µL (40-65 µg, 0.24-0.4 nmol) of StemEdit OC-1 protein to form the RNP complex, with electroporation conditions of 1200 V, 20 ms, 2 pulses.\u003c\/p\u003e\n\u003cp\u003eAfter electroporation, the cells are immediately transferred to an appropriate growth medium, and the resulting cells are screened after 48-72 hours of culture using a PCR-genotyping-based approach. The resulting sequencing data can be analyzed using the ICE Application (EditCo) to determine the editing efficiency.\u003c\/p\u003e\n\u003cp\u003eOther methods that are commonly used with Cas nuclease-based gene editing systems for forming the RNP and delivering it to the cells will probably work with OC-1 Protein as well.  Optimization of the RNP formation and delivery conditions may be required to determine conditions suitable for your gRNAs, cells, and RNP delivery system. \u003c\/p\u003e\n\u003cdiv\u003e\u003cstrong\u003eLicensing\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003eStemEdit OC-1 Protein is based on OpenCRISPR-1™ technology. The product is intended for research use only and is subject to Profluent Bio’s applicable terms of use. Commercial use of StemEdit OC-1 Protein requires a separate commercial-use agreement with Profluent Bio. Please contact REPROCELL for further information.\u003c\/div\u003e\n\u003cdiv\u003e \u003c\/div\u003e\n\u003cdiv\u003eStemEdit Hypoimmune iPSC Lines and StemEdit Genome Editing Services: The applicable license for research, clinical, and commercial use is included with the off-the-shelf hypoimmune iPSC lines or service, and the customer does not require a separate or additional license for such use, subject to the applicable terms of use and service agreement.\u003c\/div\u003e\n\u003cdiv\u003e \u003c\/div\u003e\n\u003cdiv\u003eUsers remain responsible for ensuring compliance with applicable third-party intellectual property covering guide RNAs, delivery methods, and downstream applications.\u003c\/div\u003e\n\u003cdiv\u003e \u003c\/div\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFind out about the science behind the OC-1 protein in\u003ca href=\"https:\/\/www.reprocell.com\/blog\/opencrispr-1-the-first-ai-designed-crispr-editor-for-high-precision-gene-cell-therapy\" rel=\"noopener\"\u003e our recent blog post.\u003c\/a\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnock-in of large gene fragments\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(e.g., reporters, regulatory elements)\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnock-in of biallelic mutations\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003efor disease models or functional studies\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003e\u003cspan\u003eKnock-out of single or multiple genes\u003c\/span\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eStemEdit OC-1 Protein is intended for Research Use Only.\u003c\/strong\u003e For clinical therapeutic gene editing projects, please\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.reprocell.com\/gene-editing-services\/stemedit\" rel=\"noopener\"\u003econtact our (StemEdit) service team\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003eto learn more about our genome engineering capabilities. \u003cbr\u003e\u003cbr\u003eSee also:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.reprocell.com\/product-catalog\/stemedit-gene-edited-ipscs\" rel=\"noopener\"\u003eStemEdit Gene Edited iPSCs\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e— Our hypoimmune iPSCs\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.reprocell.com\/gene-editing-services\/stemedit\" rel=\"noopener\"\u003eStemEdit Clinical Gene Editing Service\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e— Our\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003elicense-free\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eediting services.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.reprocell.com\/clinical-stem-cell-services\" rel=\"noopener\"\u003eClinical Stem Cell Services\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e— Clinical-grade iPSC solutions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/www.reprocell.com\/product-catalog\/induced-pluripotent-stem-cells\" rel=\"noopener\"\u003eStemRNA™ iPSCs\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e— Research-grade iPSC collection.\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.reprocell.com\/hs-fs\/hubfs\/OC1%20QC%20Fig1.jpg\" width=\"589\" height=\"367\"\u003e\u003cbr\u003e\u003cstrong\u003eFigure\u003c\/strong\u003e.\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eStemEdit OC-1 Protein was assayed for gene editing activity using a test guide RNA and a target cell, and the data were analyzed using the ICE App (\u003ca href=\"https:\/\/ice.editco.bio\/#\/\"\u003ehttps:\/\/ice.editco.bio\/#\/\u003c\/a\u003e). This assay showed that this batch of OC-1 achieved 80% indel formation, demonstrating robust gene editing activity.\u003c\/span\u003e\u003c\/p\u003e\n\u003chr\u003e\n\u003cp\u003e\u003cspan\u003eStemgent and the StemEdit brand name are trademarks of REPROCELL Inc., Japan. OpenCRISPR-1 is a trademark of Profluent Bio, Inc.\u003c\/span\u003e\u003c\/p\u003e","brand":"Stemgent","offers":[{"title":"130 ug","offer_id":46550023176378,"sku":"03-0020","price":600.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0764\/3119\/6346\/files\/03-0020Vial2.jpg?v=1788542035","url":"https:\/\/store.reprocell.com\/products\/stemedit-oc-1-protein","provider":"Reprocell","version":"1.0","type":"link"}