Product Name: Stemfactor FGF-basic, Human Recombinant
Catalog Number: 03-0002
Size: 50 µg
Purity: Greater than 95% by SDS-PAGE analysis
Formulation: Lyophilized from 5 mM Tris-HCl, pH 7.5 with 150 mM NaCl
Reconstitution: Centrifuge briefly and reconstitute in 500 µL of 10 mM Tris, pH 7.6, to yield 0.1 mg/mL. Avoid freeze-thaw cycles as it can result in loss of activity.
Storage and Stability: Shipped at room temperature. Lyophilized product is stable up to 6 months from date of receipt when stored at -20°C to -80°C. Reconstituted at 0.1 mg/mL is stable for up to 3 months at -20°C and up to 6 months at -80°C.
Sterility: Tested negative for Mycoplasma sp. by PCR and microbial contamination by sterility test
Source: E. coli
Amino Acid Sequence: MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Uniprot Accession Number: P09038, residues 1-155
Endotoxin Level: Less than 1.0 EU/µg as determined by the LAL method
Biologic Activity: ED50 0.1 to 1 ng/mL as determined by the dose dependent proliferation of NIH 3T3 cells
