Stemolecule A83-01
Stemgent

Stemolecule A83-01

QTY Cat # Size Unit Price
04-0014 2mg $162.00
04-0014-10 10mg $541.00

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Overview

A83-01 is a selective inhibitor of the transforming growth factor-β (TGFβ) type I receptor ALK5, the Activin/Nodal receptor ALK4, and the nodal receptor ALK71. This molecule is more potent than SB431542 in its inhibition of ALK4, 5, and 7, and only weakly inhibits ALK1, 2, 3, and 6. A83-01 inhibits the TGFβ-induced epithelial-to-mesenchymal transition (EMT) via the inhibition of Smad2 phosphorylation2.

Specifications

Product Name: Stemolecule A83-01

Catalog Number: 04-0014

Pack Size:

  • 2 mg (Cat. No. 04-0014)
  • 10 mg (Cat. No. 04-0014-10)

Alternate Name(s):

  • A-83-01
  • A 83-01
  • 3-(6-Methyl-2-pyridinyl)-N-phenyl-4-(4-quinolinyl)-1H-pyrazole-1-carbothioamide

Chemical Formula: C25H19N5S

Molecular Weight: 421.52

CAS Number: 909910-43-6

Purity: Greater than 98% by HPLC analysis

Formulation: Pale yellow solid

Solubility: A83-01 is soluble in DMSO at 50 mM.

Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C. The effect of storage of stock solutions should be verified for each application.

Quality Control: The purity of A83-01 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. No acute cytotoxicity was observed in pluripotent stem cells following a 6 hour exposure to 1 nM - 100 µM A83-01.

Documents

SDS-04-0014-04-0014-10-A83-01-v1.3
References
  1. Tojo, M., Hamashima, Y., Hanyu, A., Kajimoto, T., Saitoh, M., Miyazono, K., Node, M., and Imamura, T. The ALK-5 inhibitor A-83-01 inhibits Smad signaling and epithelial-to-mesenchymal transition by transforming growth factorβ. Cancer Sci 96: 791-800 (2005).
  2. Li, W., Wei, W., Zhu, S., Zhu, J., Shi, Y., Lin, T., Hao, E., Hayek, A., Deng, H., and Ding, S. Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. Cell Stem Cell 4: 16-19 (2009).