StemRNA 3rd Gen Reprogramming Kit
| QTY | Cat # | Size | Unit Price |
|---|---|---|---|
|
|
00-0076 | 1 kit | $1,750.00 |
* Prices listed are in USD. Taxes, shipping & handling not included. Contact us for bulk pricing.
Overview
Third Generation RNA Kit for Cellular Reprogramming of Fibroblasts, Blood, and Urine
The latest evolution in Stemgent RNA reprogramming, the StemRNA 3rd Gen Reprogramming Kit (formerly called the StemRNA-NM Reprogramming Kit), combines non-modified RNA and microRNA technology to provide a kit for stem cell researchers with a new level of versatility, simplicity, and time savings, enabling cellular reprogramming of human fibroblasts and cells from blood and now urine for difficult-to-reprogram patient samples.
Key Benefits
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Flexible technology generates high-quality human iPS cell lines from multiple target cell types
Out-of-the-box reprogramming of cells from skin (fibroblasts), blood (endothelial progenitor cells; EPCs), and urine (urine-derived progenitor cells; UPCs) -
High-efficiency, non-integrating reprogramming
StemRNA 3rd Gen requires a few as four additional reagents. Double-stranded microRNAs enhance reprogramming efficiency, providing iPS cells with high efficiency (up to 4% from fibroblasts, up to 3% from EPCs, up to 0.5% from UPCs). -
Time-saving protocol delivers faster results, facilitating higher throughput
Colonies ready to pick in 10-14 days from fibroblasts and 12-16 days from EPCs or UPCs. No screening needed
| StemRNA 3rd Gen Reprogramming Kit (00-0076) | |||
|---|---|---|---|
| Feature | Fibroblasts | Urine (UPCs) | Blood (EPCs) |
| No. wells per kit | 9 | 3 | 3 |
| No Transfections required | 4 | 6-8 | 6-8 |
| Days to primary iPS cell colonies | 10-14 | 12-14 | 12-14 |
| Reprogramming efficiency | 2-4% | 0.1-0.5% | 0.4-3% |
| Screening Required | No | No | No |
| Xeno-compatible protocol | Yes | No | No |
P7 primary iPS cells derived from UPCs were stained using the StainAlive TRA-1-60 Antibody ( Cat. No. 09‑0068 ) and DAPI after expansion in NutriStem hPSC XF Medium ( Cat. No. 01‑0005)
iPS cells derived from fibroblasts using the StemRNA 3rd Gen Reprogramming Kit were cultured on iMatrix-511 (Cat. No. NP892‑011) in NutriStem hPSC XF Medium ( Cat. No. 01‑0005 ) for 7 passages. Magnification: 4x
Timeline for StemRNA 3rd reprogramming of fibroblasts