StemRNA 3rd Gen Reprogramming Kit

Third Generation RNA Kit for Cellular Reprogramming of Fibroblasts, Blood, and Urine The latest evolution in Stemgent RNA reprogramming, the StemRNA 3 rd Gen Reprogramming Kit (formerly called the StemRNA-NM Reprogramming Kit), combines non-modified RNA and microRNA technology to provide a kit for stem cell researchers with a new level of versatility, simplicity and time savings, enabling cellular reprogramming of human fibroblasts, and cells from blood and now urine for difficult to reprogram patient samples. Key Benefits Flexible technology generates high-quality human iPS cell lines from multiple target cell types Out-of-the-box reprogramming of cells from skin (fibroblasts), blood (endothelial progenitor cells; EPCs) and urine (urine-derived progenitor cells; UPCs) High efficiency, non-integrating reprogramming StemRNA-3 rd Gen requires a few as four additional reagents. Double stranded microRNAs enhance reprogramming efficiency, providing iPS cells with high efficiency (up to 4% from fibroblasts, up to 3% from EPCs, up to 0.5% from UPCs). Time-saving protocol delivers faster results facilitating higher throughput Colonies ready to pick in 10-14 days from fibroblasts and 12-16 days from EPCs or UPCs. No screening needed StemRNA-3 rd Gen Reprogramming Kit (00-0076) Feature Fibroblasts Urine (UPCs) Blood (EPCs) No. wells per kit 9 3 3 No Transfections required 4 6-8 6-8 Days to primary iPS cell colonies 10-14 12-14 12-14 Reprogramming efficiency 2-4% 0.1-0.5% 0.4-3% Screening Required No No No Xeno-compatible protocol Yes No No P7 primary iPS cells derived from UPCs were stained using the StainAlive TRA-1-60 Antibody ( Cat. No. 09‑0068 ) and DAPI after expansion in NutriStem hPSC XF Medium ( Cat. No. 01‑0005 ) iPS cells derived from fibroblasts using the StemRNA-3 rd Gen Reprogramming Kit were cultured on iMatrix-511 (Cat. No. NP892‑011) in NutriStem hPSC XF Medium ( Cat. No. 01‑0005 ) for 7 passages. Magnification: 4x Timeline for StemRNA 3 rd reprogramming of fibroblasts Download our StemRNA-3 rd Gen Reprogramming Posters: ISSCR 2015 ISSCR 2016