Stemgent Sox2 mRNA encodes the Sox2 protein commonly used in cellular reprogramming systems for the generation of induced pluripotent stem (iPS) cells. This in vitro transcribed mRNA incorporates both pseudouridine and 5-methylcytidine modified nucleotides to minimize the cellular interferon response to single stranded RNA.1,2Repeated transfection of Sox2 mRNA in combination with mRNAs encoding Oct4, Klf4, Lin-28 and c-Myc has been shown to reprogram human fibroblasts.2
Figure 1: Protein expression and localization shown by immunocytochemistry, DAPI in blue and Sox2 in red.
Sox2 mRNA, Human
100 ng/ul in RNase-free TE buffer, pH 7.0
Storage and Stability
Store at or below −70 °C. Stable for a minimum of 3 months from date of receipt when stored as directed. Wear gloves when handling this product. Use RNase-free tubes and barrier pipette tips. Thaw on ice. Keep on ice until the sample is removed. Avoid multiple freezing and thawing cycles (<5). Aliquot to experimental quantities.
Proper protein expression and localization have been confirmed by immunocytochemistry.
Notice To Purchaser
This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic and prognostic use, (iii) any use in delivery to and/or modification of cells that are intended for clinical, diagnostic or medicinal use, including without limitation, cell-based therapy, (iv) any commercial purposes, including without limitation the performance of contract research or provision of services to a third party and the manufacture of products for general sale. Any use of this product for any of the abovementioned purposes requires a license from the Massachusetts Institute of Technology.
Stemgent Sox2 mRNA, Human should be transfected using a protocol specifically optimized for the cell type used in the experiment.