The latest evolution in Stemgent RNA reprogramming, the StemRNA 3rd Gen Reprogramming Kit (formerly called the StemRNA-NM Reprogramming Kit), combines non-modified RNA and microRNA technology to provide a kit for stem cell researchers with a new level of versatility, simplicity and time savings, enabling cellular reprogramming of human fibroblasts, and cells from blood and now urine for difficult to reprogram patient samples.
|StemRNA-3rd Gen Reprogramming Kit (00-0076)|
|Feature||Fibroblasts||Urine (UPCs)||Blood (EPCs)|
|No. wells per kit||9||3||3|
|No Transfections required||4||6-8||6-8|
|Days to primary iPS cell colonies||10-14||12-14||12-14|
P7 primary iPS cells derived from UPCs were stained using the StainAlive TRA-1-60 Antibody (Cat. No. 09‑0068) and DAPI after expansion in NutriStem hPSC XF Medium (Cat. No. 01‑0005)
iPS cells derived from fibroblasts using the StemRNA-3rd Gen Reprogramming Kit were cultured on iMatrix-511 (Cat. No. NP892‑011) in NutriStem hPSC XF Medium (Cat. No. 01‑0005) for 7 passages. Magnification: 4x
Timeline for StemRNA 3rd reprogramming of fibroblasts
Download our StemRNA-3rd Gen Reprogramming Posters:
StemRNA 3rd Gen Reprogramming Kit
Storage and Stability
Store all three kit components at or below −70 °C. Kit components are stable for a minimum of 3 months from date of receipt when stored as directed.
The individual mRNAs are tested for size and integrity. The StemRNA 3rd Gen Reprogramming Kit is functionally validated for successful RNA-based reprogramming of adult fibroblasts, human umbilical vein endothelial cells (HUVECs), EPCs, and UPCs. Complete reprogramming of iPS cell colonies is confirmed by expression of pluripotency markers and appropriate colony morphology. All components of the kit are sterile and have tested negative for Mycoplasma spp.
For use with associated StemRNA 3rd Gen Reprogramming Kit protocols for generation of iPS cells.
Gagliano O; Luni C; Qui W; Bertin E; Torchio E; Galvanin S; Urciuolo A; Elvassore N. Microfluidic reprogramming to pluripotency of human somatic cells. Nat. Protocols 14:772-737 (2019).
The StemRNA 3rd Gen Reprogramming Kit was used to generate iPSCs from fibroblasts in a microfluidic system that used only 1500 cells in 20 µL of medium. RNA-based reprogramming is ideal for such an application since it does not require extended culture for the elimination of reprogramming vectors.