The aminopyrimidine CHIR99021 is the most selective inhibitor of glycogen synthase kinase 3 β (GSK-3β) reported to date1,2. Unlike other potent inhibitors of GSK-3, CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3β compared to CDKs3. Along with the elimination of differentiation-inducing signaling from mitogen-activated protein kinases, using CHIR99021 to block the activity of GSK-3β enables the self-renewal of embryonic stem cells4.
Also available as a convenient 10 mM solution in DMSO (Cat. No. 04-0004-02)
Product Name
Stemolecule CHIR99021
Catalog Number
04-0004
Size
2 mg (Cat. No. 04-0004)
10 mg (Cat. No. 04-0004-10)
2 mg in 10 mM DMSO solution (Cat. No. 04-0004-02)
AlternateName(s)
6-[[2-[[4-(2,4-dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2 pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile
Chemical Formula
C22H18Cl2N8
Molecular Weight
465.34
CAS Number
252917-06-9
Purity
Greater than 95% by HPLC analysis
Formulation
Off-white solid
Solubility
Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet.
Storage and Stability
Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C. The effect of storage of stock solutions should be verified for each application.
Quality Control
The purity of CHIR99021 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of CHIR99021 was tested on mouse embryonic stem cells.
