Stemolecule Doxycycline hyclate
Stemgent

Stemolecule Doxycycline hyclate

QTY Cat # Size Unit Price
04-0016 10 mg $70.00

* Prices listed are in USD. Taxes, shipping & handling not included. Contact us for bulk pricing.

Add to My Quote
Have questions? Message Us

Overview

Stemolecule Doxycycline hyclate (dox) is a broad-spectrum antibiotic derivative of tetracycline and an inhibitor of matrix metalloproteinases1. Tetracycline-controlled transcriptional activation is a method of inducible expression whereby transcription is reversibly turned on or off in the presence of tetracycline or one of its derivatives, such as dox2. For this type of reprogramming method, dox-inducible lentiviral reagents are used to induce the expression of virally transduced genes and generate induced pluripotent stem (iPS) cells from somatic cells3-7.

This product is intended for Research Use Only.

Documents

Sds stemgent 04 0016 d001002 doxycyclinehyclate
References
  1. Burgraff, D., Trinkl, A., Dichgans, M., and Hamann, G.F. Doxycycline inhibits MMPs via modulation of plasminogen activators in focal cerebral ischemia. Neurobiol Dis 25: 506-513 (2007).
  2. Bujard, H., and Gossen, M. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci 89: 5547-5551 (1992).
  3. Brambrink, T., Foreman, R., Welstead, G.G., Lengner, C.J., Wernig, M., Suh, H., and Jaenisch, R. Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2: 151-159(2008).
  4. Wernig, M., Lengner, C.J., Hanna, J., Lodato, M.A., Steine, E., Foreman, R., Staerk, J., Markoulaki, S., and Jaenisch, R. A drug-inducible transgenic system for direct reprogramming of multiple somatic cell types. Nat Biotechnol 26: 916-924 (2008).
  5. Hockemeyer, D., Soldner, F., Cook, E.G., Gao, Q., Mitalipova, M., and Jaenisch R. A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Cell Stem Cell 3: 346-353 (2008).
  6. Welstead, G.G., Brambrink, T., and Jaenisch, R. Generating iPS cells from MEFS through forced expression of Sox-2, Oct-4, c-Myc, and Klf4. J Vis Exp 7: 734 (2008).
  7. Markoulaki, S., Hanna, J., Beard, C., Carey, B.W., Cheng, A.W., Lengner, C.J., Dausman, J.A., Fu, D., Gao, Q., Wu, S., Cassady, J.P., and Jaenisch, R. Transgenic mice with defined combinations of drug-inducible reprogramming factors. Nat Biotechnol 27: 169-171 (2009).