Stemolecule LY411575 is a selective, cell permeable, small molecule gamma secretase inhibitor which blocks Notch activation1. Notch signaling is a pathway known to regulate both differentiation and proliferation of cells in diverse adult tissues. LY411575 has been shown to promote goblet cell differentiation in mice intestine. Concurrently, LY411575 inhibits cell proliferation2. LY411575 has also been observed to promote neural differentiation of mouse embryonic stem (ES) cells3.
Greater than 96% by HPLC analysis
For a 10 mM concentrated stock solution of LY411575, reconstitute the compound by adding 1.04 mL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 100 mM.
Storage and Stability
Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
The purity of LY411575 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of LY411575 was tested on mouse embryonic stem cells.