Stemgent Stemolecule SB431542

SB431542 is an inhibitor of the transforming growth factor-β 1 (TGFβ1) activin receptor-like kinases (ALKs). It is a selective and potent inhibitor of ALK-4, -5 and -7. SB431542 inhibits endogenous activin and TGFβ signaling without affecting more divergent BMP signaling utilizing ALK-1, -2, -3, and -6^1,2. SB431542 stimulates proliferation, differentiation, and sheet formation of endothelial cells derived from embryonic stem cells³.

Also available: Stemolecule SB431542 in Solution (Cat, No. 04-0010-05)

Product Name

Stemolecule SB431542

Catalog Number

04-0010-base

Size

• 10 mg (Cat. No. 04-0010-10)
• 5 mg (Cat. No. 04-0010)
• 5 mg at 10 mM in DMSO (04-0010-05)

Alternate Name(s)

4-[4-(1,3-benzodioxol-5-yl)-5-pyridin-2-yl-1H-imidazol-2-yl]benzamide

Chemical Formula

C₂₂H₁₆N₄O₃

Molecular Weight

384.4

CAS Number

301836-41-9 (anhydrous)

Purity

Greater than 98% by HPLC analysis

Formulation

White solid

Solubility

Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet.

Storage and Stability

Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.

Quality Control

The purity of SB431542 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of SB431542 was tested on mouse embryonic stem cells.

Specification Sheets

• 04-0010 Specifications Sheet
• 04-0010-10 Specifications Sheet
• 04-0010-05 Specifications Sheet

Safety Data Sheets

• 04-0010 and 04-0010-10 Safety Data Sheet (SDS)
• 04-0010-05 Safety Data Sheet (SDS)

1. Matsuyama, S., Iwadate, M., Kondo, M., Saitoh, M., Hanyu, A., Shimizu, K., Aburatani, H., Mishima, H.K., Imamura, T., Miyazono, K., Miyazawa, K. SB-431542 and Gleevec inhibit transforming growth factor-β-induced proliferation of human osteosarcoma cells. Cancer Res 63: 7791-7798 (2003).
2. Laping, N.J., Grygielko, E., Mathur, A., Butter, S., Bomberger, J., Tweed, C., Martin, W., Fornwald, J., Lehr, R., Harling, J., Gaster, L., Callahan, J.F., and Olson, B.A. Inhibition of transforming growth factor (TGF)-β1-induced extracellular matrix with a novel inhibitor of the TGF-β type I receptor kinase activity: SB-431542. Mol Pharmacol 62: 58-64 (2002).
3. Inman, G.J., Nicolás, F.J., Callahan, J.F., Harling, J.D., Gaster, L.M., Reith, A.D., Laping, N.J., and Hill, C.S. SB-431542 is a potent and specific inhibitor of transforming growth factor-β superfamily type I activin receptor-like kinase (ALK) receptors ALK4, ALK5, and ALK7. Mol Pharmacol 62: 65-74 (2002).
4. Watabe, T., Nishihara, A., Mishima, K., Yamashita, J., Shimizu, K., Miyazawa, K., Nishikawa, S., and Miyazono, K. TGFβ receptor kinase inhibitor enhances growth and integrity of embryonic stem cell-derived endothelial cells. J Cell Biol 163: 1303-1311 (2003).

Additional Publications

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