Stemolecule Wnt Inhibitor IWP-3
Stemgent

Stemolecule Wnt Inhibitor IWP-3

QTY Cat # Size Unit Price
04-0035 2 mg $135.00

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Overview

Stemolecule Wnt Inhibitor IWP-3 was identified in a high-throughput screen for antagonists of the Wnt/β-catenin pathway. Wnt Inhibitor IWP-3 prevents palmitoylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin1. The inhibition of the Wnt pathway through the use of IWP-3 has also been shown to promote the formation of cardiomyocytes from human embryonic stem cell-derived mesoderm cells1.

Specifications

Product Name: Stemolecule Wnt Inhibitor IWP-3

Catalog Number: 04-0035

Size: 2 mg

Alternate Name(s): 2-(3-(4-fluorophenyl)-3,4,6,7-tetrahydro-4-oxothieno[3,2-d]pyrimidin-2-ylthio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide

Chemical Formula: C22H17FN4O2S3

Molecular Weight: 484.59

CAS Number: 687561-60-0

Purity: Greater than 99 % by HPLC analysis

Formulation: Light yellow solid

Solubility: This molecule is soluble in DMSO at 100 mM.

Reconstitution: For a 10 mM concentrated stock solution of Wnt Inhibitor IWP-3, reconstitute the compound by adding 412.7 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, prewarm the medium prior to adding reconstituted compound. Note: For most cell culture applications, the total DMSO concentration should be kept below 0.5%.

Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.

Quality Control: The purity of Wnt Inhibitor IWP-3 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Wnt Inhibitor IWP-3 was tested on mouse embryonic stem cells.

Documents

Sds 04 0035 iwp3 v1.2
References
  1. Chen, B., Dodge, M.E., Tang, W., Lu, J., Ma, Z., Fan, C.W., Wei, S., Hao, W., Kilgore, J., Williams, N.S., Roth, M.G., Amatruda, J.F., Chen, C., and Lum, L. Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat Chem Biol 5: 100-107 (2009).
  2. Willems, E., Spiering, S., Davidovics, H., Lanier, M., Xia, Z., Dawson, M., Cashman, J., and Mercola, M. Small-molecule inhibitors of the Wnt pathway potently promote cardiomyocytes from human embryonic stem cell-derived mesoderm. Circ Res 109: 360-364 (2011).