Stemolecule XAV939 is a cell-permeable small molecule inhibitor of the Wnt / β-catenin pathway. XAV939 inhibits tankyrase 1 and tankyrase 2, thus stabilizing axin and stimulating β-catenin degradation1. Current research suggests that Wnt proteins act to maintain stem cells in an undifferentiated, self-renewing state2. Wnt proteins act on a variety of stem cells that include neural, mammary and embryonic stem cells. In addition, XAV939 inhibits growth of DLD-1 cells, an APC-deficient colorectal cancer cell line1.
Product Name
Stemolecule XAV939
Catalog Number
04-0046
Size
2 mg
AlternateName(s)
2-(4-(trifluoromethyl)phenyl)-7,8-dihydro-5H-thiopyrano[4,3-d]pyrimidin-4-ol
Chemical Formula
C14H11F3N2OS
Molecular Weight
312.31
CAS Number
284028-89-3
Purity
Greater than 95% by HPLC analysis
Formulation
White powder
Solubility
XAV939 is reported to be soluble in DMSO at 100 mM.
Reconstitution
For a 10 mM concentrated stock solution of XAV939, reconstitute the compound by adding 640.4 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For use in cell culture, warm the media prior to adding reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%.
Storage and Stability
Store powder at −20 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
Quality Control
The purity of XAV939 is determined by HPLC analysis. The molecular weight is determined by mass spectrometry. No acute cytotoxicity is observed in mouse embryonic stem cells following a 6 hour exposure to 1 nM — 100 uM of XAV939.
