Stemgent Stemolecule Y27632

Y27632 is a cell-permeable small molecule Rho-associated kinase (ROCK) inhibitor¹. Y27632 has been found to prevent apoptosis as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency². This molecule has also been shown to enhance survival during the transplantation of ES cell-derived neural precursors³. Y27632 in combination with Pifithrin-u significantly improves cell recovery after cryopreservation⁴.

Stemolecule Y27632 is also available as a 10 mM DMSO Stock Solution (Cat. No. 04-0012-02).

Y27632 is widely used in naive stem cell protocols⁵, such as the 5i/L/A/ protocol.

Product Name

Stemolecule Y27632

Catalog Number

04-0012

Size

2 mg (Cat. No. 04-0012)

10 mg (Cat. No. 04-0012-10)

2 mg in 10 mM DMSO (Cat. No. 04-0012-02)

Alternate Name(s)

• Y-27632
• (1R,4R)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexanecarboxamide dihydrochloride

Chemical Formula

C₁₄H₂₁N₃O·2HCl

Molecular Weight

320.3

CAS Number

146986-50-7

Purity

Greater than 97% by HPLC analysis

Formulation

White solid

Solubility

Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet.

Storage and Stability

Store powder at 4 °C protected from light. Following reconstitution, store aliquots at −20 °C. Stock solutions are stable for 6 months when stored as directed.

Quality Control

The purity of Y27632 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Y27632 was tested on mouse embryonic stem cells.

Specification Sheets

• 04-0012 Specifications Sheet
• 04-0012-10 Specifications Sheet
• 04-0012-02 Specifications Sheet

Safety Data Sheets

• 04-0012 and 04-0012-10 Safety Data Sheet
• 04-0012-02 Safety Data Sheet

1. Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57: 976-983 (2000).
2. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K., and Sasai, Y. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25: 681-686 (2007).
3. Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. J Neurosci Res 86: 270-280 (2008).
4. Xu, X., Cowley, S., Flaim, C.J., James, W., Seymour, L.W., Cui, Z. Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation. Biotechnol Prog 26: 781-788 (2010).
5. Fonseca, SAS; Costas, RM; Pereira, LV. Searching for naive human pluripotent stem cells. World Journal of Stem Cells, 7(3), 649 (2015).

Additional Publications

• Li Y; Li J; Wang J; Zhang S; Giles K; Prakash TP; Rigo F; Napierala JS; Napierala M. Premature transcription termination at the expanded GAA repeats and aberrant alternative polyadenylation contributes to the Frataxin transcriptional deficit in Friedreich’s ataxia. Human Molec Genetics 31:3539 (2022).
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• Carberry BJ; Hergert JE; Yavitt FM; Hernandez JJ; Speckl KF; Bowman CN; McLeod RR; Anseth KS. 3D printing of sacrificial thioester elastomers using digital light processing for templating 3D organoid structures in soft biomatrices. Biofabrication 13:044104 (2021).
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• Schoger E; Zimmermanm W-H; Cyganek L Zalarayán LC. Establishment of a second generation homozygous CRISPRa human induced pluripotent stem cell (hiPSC) line for enhanced levels of endogenous gene activation. Stem Cell Res 56:102518 (2021).
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• Rajput SA; Liang S-J; Wang W-Q; Yan H-C. Lycopene Protects Intestinal Epithelium from Deoxynivalenol-Induced Oxidative Damage via Regulating Keap1/Nrf2 Signaling. Antioxidants 10:1493 (2021).
• Meshrkey F; Ayuso AC; Rao RR; Iyer S. Quantitative analysis of mitochondrial morphologies in human induced pluripotent stem cells for Leigh syndrome. Stem Cell Res 57:102572 (2021).
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• Chen D; Sun N; Hou L; Kim R; Faith J; Aslanyan M; Tao Y; Zheng Y; Fu J; Liu W; Kellis M; Clark A. Human Primordial Germ Cells Are Specified from Lineage-Primed Progenitors. Cell Rep 29:4568-4582.e5 (2019).
• Koutmani Y; Gampierakis IA; Polissidis A; Ximerakis M: Koutsoudaki PN; Polyzos A; Agrogiannis G; Karaliota S; Thomaidou D; Rubin LL; Politis PK; Karakis KP. CRH Promotes the Neurogenic Activity of Neural Stem Cells in the Adult Hippocampus. Stem Cell Rep 28:932-945.e7 (2019).
• Nagaraja S; Quezada MA; Gillespie SM; Arzt M; Lennon JJ; Woo PJ; Hovestadt V; Kambhampati M; Filbin MG; Suva ML; Nazarian J; Monje M. Histone Variant and Cell Context Determine H3K27M Reprogramming of the Enhancer Landscape and Oncogenic State. Molecular Cell 76:dev.138982 (2019).
• Trautsch I; Heta E; Soong PL; Levent E; Nikolaev VO; Bogeski I ; Katschinski DM; Mayr M; Zimmermann W-H. Optogenetic Monitoring of the Glutathione Redox State in Engineered Human Myocardium. Front Physiol 10:272 (2019).
• LI S; Zhang H; Wang A; Liu Y; Yue F; Abulati X; Zhang C; Li L. Differentiation of adult human retinal pigment epithelial cells into dopaminergic-like cells in vitro and in the recipient monkey brain. Molecular Med 25:9 (2019).
• Talbot NC; Shannon AE; Garrett WM. Pancreatic duct-like cell line derived from pig embryonic stem cells: Expression of uroplakin genes in pig pancreatic tissue. In vitro Cell Develop Biol - Animal 2019:1 (2019).
• Wadhwani AR; Affaneh A; Van Gulden S; Kessler JA. Neuronal Apolipoprotein E4 increases cell death and p‐tau release in Alzheimer's disease. Ann Neurol doi: 10.1002/ana.25455.: (2019).
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• Dai S-B; Shen T; Zheng T-T; Pu J-L; Chen X-Z. Plasmid-based generation of neural cells from human fibroblasts using non-integrating episomal vectors. Neural Regen Res 14:510-505 (2019).
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• Chen D; Liu W: Zimmerman J; Pastor WA; Kim R;  Hosohama L; Ho J; Aslanyan M; Gell JJ; Jacobsen SE; Clark AT. The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation. Cell Reports 25:3591-3602.e5 (2018).
• Worley KE; Chin AS; Wan LQ. Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation. Stem Cells Intl 2018:1848605 (2018).
• Kilander MB; Wang C-H; Chang C-H; Nestor JE; Herold K; Tsai J-W; Nestor MW; Lin Y-C. A rare human CEP290 variant disrupts the molecular integrity of the primary cilium and impairs Sonic Hedgehog machinery. Sci Rep 8:17335 (2018).
• Javed AO; Li Y; Muffat J; Su K-C; Cohen MA; Lungjangwa T; Auborg P; Cheeseman IM; Jaenisch R. Microcephaly Modeling of Kinetochore Mutation Reveals a Brain-Specific Phenotype. Cell Rep 25:368-382.e5 (2018).
• Memon B; Karam M; Al-Khawaga S; Abdelalim EM. Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1. Stem Cell Res Therap 9:15 (2018).
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• Donegan JJ; Tyson JA; Branch SY; Beckstead MJ; Anderson SA; Lodge DJ. Stem cell-derived interneuron transplants as a treatment for schizophrenia: preclinical validation in a rodent model. Mol Psychiatry 22:1942-1501 (2017).
• McCracken KW; Aihara E; Martin B; Crawford CM; Broda T; Treguier J; Zhang X; Shannon JM; Montrose MH; Wells JM. Wnt / β-catenin promotes gastric fundus specification in mice and humans. Nature 541:182-187 (2017).
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