REPROCELL ReproFF2

Weekend-free culture of pluripotent stem cells

ReproFF2 is a new feeder-free culture medium for human ES/iPS cells. Unlike conventional stem cell media that requires changing on a daily basis, ReproFF2 needs culture maintenance only three days a week. Change the medium on Monday and Wednesday, passage the cells on Friday. Consequently, the cost and amount of medium used are halved, and feeder cells need not be prepared. Also, the culture can remain unattended during the weekend.

ReproFF2 offers:

• Culture maintenance required only three days/week.
• Reduce the labor cost for the weekend.
• Maintains undifferentiated state and differentiation capability.
• Reduced need for mechanical manipulation for passaging.

ReproFF2 has been developed to reduce the steps in the workflow. Just warm up, add bFGF*, and the medium is ready to use.

Product Name

ReproFF2 Cell Culture Medium

Catalog Number

RCHEMD006

Size

500 mL

Formulation

Contains minute amounts of β-mercaptoethanol

Storage and Stability

Store basal medium at -20 °C. Medium is stable for 3 years when stored as directed.

Quality Control

• Each lot is culture-tested with human iPS cells as described in Takahashi K, et al., Cell 2007;131:861–72.
• Lot-to-lot control testing of other critical criteria, including osmolality, pH, sterility, and Mycoplasma is performed.

Notice To Purchaser

This product is for research use only, not for therapeutic or diagnostic purposes.Not for sale to a third party or for use for commercial purposes without permission from ReproCELL.

Recommended Usage

Serum-free, requires addition of bFGF

Manual

• Instruction Manual

Safety Data Sheet

• ReproFF2 Safety Data Sheet