The StemAb RNA Transfection Kit is a proprietary mixture of lipid components is specifically designed for in vitro RNA transfection. This kit has been shown to deliver mRNA, siRNA and miRNA. StemAb RNA Transfection Kit provides greater than or equal to 90% transfection efficiency of mRNA into a range of cell types with greater than 95% viability; these cell types include human embryonic stem (hES) cells, Jurkat cells, human fibroblasts, and HEK293T-cells. StemAb RNA Transfection Kit has been successfully used to deliver RNA to human ES cells, dendritic cells, and retinal pigment epithelial cells. There is no need to serum-starve cells using this kit as the transfection efficiency remains greater than or equal to 90% in the presence of serum.
Higher levels of protein expression with StemFect RNA Transfection Kit. BJ fibroblasts were transfected with GFP mRNA. GFP expression was assessed at 18 h post-transfection.
Titrate protein expression by modifying the amount mRNA used for transfection. BJ fibroblasts were transfected with eGFP mRNA. Transfection efficiency and GFP intensity were determined at 18 h post-transfection.
High efficiency delivery of siRNA to hES cells. HUES9 cells were transfected with siRNA using StemAb RNA Transfection Kit. (A) Greater than 90% of hES cells were transfected with FAM labeled control siRNA, 75 nM (250 ng).
High efficiency delivery of siRNA to hES cells. HUES9 cells were transfected with siRNA using StemAb RNA Transfection Kit. (C) Transfection with 10nM and 20nM Oct4 siRNA results in altered colony morphology.
High efficiency delivery of siRNA to hES cells. HUES9 cells were transfected with siRNA using StemAb RNA Transfection Kit. (B) Transfection with Oct4 siRNA, 20 nM (250 ng), resulted in altered colony morphology and expression of SSEA-1 indicating differentiation.
Human embryonic stem (hES) cells remain undifferentiated after transfection. H1 hES cells cultured in NutriStem were transfected with eGFP mRNA using StemAb RNA Transfection Kit. (A) Greater than 95% of cells maintained pluripotency marker expression, SSEA-4, 24 hours post-transfection.
Human embryonic stem (hES) cells remain undifferentiated after transfection. H1 hES cells cultured in NutriStem were transfected with eGFP mRNA using StemAb RNA Transfection Kit. (B) Normal colony morphology is maintained 20 hours post-transfection.
