REPROCELL SL10 Feeder Cells

Mouse fibroblast feeder cells applicable for culture of murine, primate, human ES/iPS cells. Neomycin resistance offers a useful selection tool in transfection and drug development experiments.

This feeder cell is verified to be applicable for a wide range of ES/iPS cell culture. The figure below shows well growth of ES cells on SL10 feeder cell. The bias between lots is lower than primary MEF, which is the advantage of SL10. Detailed information is available in the protocol.

• A feeder cell line applicable for culture of murine, primates, human ES/iPS cells
• With the resistance of Neomycin which helps the selection in transfection and drug developing experiments
• Derived from the same cell line, STO, which was the feeder cell, SNL, utilized in Dr. Yamanaka’s experiment of human iPS cells establishment
• Also utilized as the feeder cell in the establishment of 3 Japanese ES cell lines (KhES-1, KhES-2, KhES-3) and Macaca fascicularis ES cell line
• Every lot has been examined before moved into inventory
• Mitomycin C-treated cells, which is ready for use

Protocol

• SL10 Feeder Cells Protocol

Data Sheets

• Lot: 20160226
• Lot: 20150716
• Lot: A01OY07
• Lot: A01OM05
• Lot: A01OJ13

Additional Publications

• Kuroda K; Komori T; Ishabashi J; Uto T; Kobayashi I; Kadokawa R; Kato Y; Ninomiya K; Takahashi K; Hirata E. Non-aqueous, zwitterionic solvent as an alternative for dimethyl sulfoxide in the life sciences. Communications Chem 3:163 (2020).