CI-huArlo Immortalized Human Alveolar Epithelial Cells
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CI-huArlo Immortalized Human Alveolar Epithelial Cells

QTY Cat # Size Unit Price
INS-CI-1031 1 vial (500 K cells) $9,430.00

* Prices listed are in USD. Taxes, shipping & handling not included. Contact us for bulk pricing.

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Overview

Immortalized human alveolar epithelial cells.

CI-huArlo cells are immortalized human alveolar epithelial cells. The cell line is an improved version of CI-hAELVi alveolar epithelial cell line. It was derived from CI-huALEVi by single-cell printing, and it shows an overall improved alveolar phenotype, most notably a tighter barrier function. 

While the cells do show an AT-1 phenotype in standard culture conditions, they adopt certain AT-2 characteristics in extended ALI culture conditions, including secretion of surfactants and formation of microvilli and lamellar structures.

Characteristics

  • TEER of >1500 Ω/sqcm in Liquid-Liquid-Interface culture
  • TEER of >2000Ω/sqcm in Air-Liquid-Interface culture
  • Tight junctions (ZO1, Occludin)
  • Monolayer growth on semipermeable Inserts.
  • Susceptible to SARS-CoV-2 infection

Culture Conditions

  • Culture Medium: huAEC Medium Kit for Alveolar Cells (Cat No. INS-ME-1013)
  • Culture Matrix: Coating Solution (Cat. No. INS-SU-1026-100ml)
  • Cryopreservation Medium: Freezing Solution for Immortalized Cell Lines (Cat. No. INS-SU-1027)

Buying and Ordering the Cells

A license for Academic Researchers is also available.  Please contact us for more information

Specifications

Product Name

CI-huArlo

Product Description

Immortalized Human Alveolar Epithelial Cells

Strain

CI-huArlo

Catalog Number

INS-CI-1031

Donor Information

  • Not available

Precryopreservation Culture Conditions

  • Culture Medium: huAEC Medium Kit for Alveolar Cells (Cat No. INS-ME-1013)
  • Culture Matrix: huAEC Coating  (Cat. No. INS-SU-1018-100ml)
  • Cryopreservation Medium: Freezing Solution for Immortalized Cell Lines (Cat. No. INS-SU-1004)
  • BSL 1

Size

1 vial, 0.5 × 106 cells per vial

Storage

Store in liquid nitrogen vapor phase.

Quality Control

  • ICC for occludin, ZO-1: Pass
  • TEER: >1000 Ω×cm2
  • Viability: >80%
  • Bacteria: Negative
  • Fungi: Negative
  • Mycoplasma: Negative
  • Virus (HIV1&2, HBV, HCV): Negative

Sterility

Sterile

Notice To Purchaser

This product is intended for Research Use Only.