iMatrix-111's features make it an ideal matrix for some cell culture applications:
iMatrix-111 is a highly purified and refined product of human recombinant laminin-111 (E8 fragment) expressed by CHO-S cells. Laminin 111 supports the survival, proliferation, and differentiation of many different cell types in vitro. However, its distribution after birth is restricted to only a few tissues, such as the brain and kidney. Many of the cultivated cell types would not encounter this protein naturally in vivo and it is advisable to match your specific cell type to the laminin naturally expressed by this cell, improving the cell culture. During development, Riechert’s membrane supporting the extraembryonic trophoblasts express laminin 111. Reichert’s membrane is essential for epiblast differentiation, which is essential for the formation of the three embryonic germ layers by pluripotent cells in the inner cell mass.
In the adult, laminin α1 chain expression is relatively restricted and is only present in some epithelial basement membranes, and primarily found in tissues such as the eye, liver, and kidney, whereas hardly expressed at all in endothelial, adipose, nervous, or muscle tissues.
Product Name
iMatrix-111 Cell Culture Substrate
Catalog Number
NP892-071
NP892-072
Size
Molecular Weight
150 kDa
Purity
>95% pure
Formulation
Purified Laminin-111 E8 proteolytic fragment
Storage and Stability
Store at 4 °C and protect from light exposure. Stable for 2 years from manufacturing date.
Quality Control
Activity: Kd for integrin binding
Sterility
Sterile
Notice To Purchaser
REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market.
Recommended Usage
iMatrix-111 is suitable for use as a substrate for culture of various cell types.
Concentration
500 µg/mL
Source
CHO-S cell expression
Manufacturer
Matrixome Corporation (Japan)
